类风湿性关节炎患者Th1-Th2、Tc1-Tc2细胞检测及意义.docVIP

类风湿性关节炎患者Th1/Th2、Tc1/Tc2细胞检测及意义 　【关键词】 类风湿性关节炎；T细胞亚群；三色流式细胞术风湿性关节炎 　　［摘 要］ 目的：探讨类风湿性关节炎(rheumatoid arthritis，RA)患者外周血Th1/Th2、Tc1/Tc2细胞亚群极化状态，分析RA的免疫学发病机制，为RA的临床治疗提供理论依据。方法：收集RA患者及健康者外周抗凝静脉血，分离纯化淋巴细胞。FITC标记的抗CD3单抗，Cy5标记的抗CD4、CD8单抗，PE标记的抗CD30单抗，以CD3/CD4、CD3/CD8设门作三色流式细胞术(Triscolor flow cytometry)，分析RA患者T细胞亚群极化状态。结果：与正常对照相比，RA患者外周血CD4+细胞百分率显著升高(P＜0.01)，CD8+细胞百分率则无明显变化(P＞0.05)，CD4+/CD8+比例明显升高(P＜0.05)；CD3＋CD4＋CD30-细胞百分率、CD4＋CD30－/CD4＋CD30＋和CD8＋CD30－/CD8＋CD30＋比例均明显升高(P＜0.01)，而CD3＋CD4＋CD30＋、CD3＋CD8＋CD30－及CD3＋CD8＋CD30＋细胞百分率无明显变化(P＞0.05)。结论：RA患者外周血存在细胞免疫功能失调，T细胞亚群极化状态发生改变，呈明显的Th1类细胞优势；Th1/Tc1型细胞介导的细胞免疫可能与RA的发病机制有关。 　　 　　［关键词］ 类风湿性关节炎；T细胞亚群；三色流式细胞术 　　Detection of the Th1/Th2、Tc1/Tc2 Subsets in Rheumatoid Arthritis Patients and Its Significance 　　Abstract：Objective To investigate the polarization of T cells subset and analyze the immunologic pathogenesis in RA.Methods The ratios of CD4+/CD8+,CD3+CD4+CD30-/CD3+CD4+CD30+ and CD3+CD8+CD30-/CD3+CD8+CD30+ in peripheral blood T cells were analyzed by immunofluorescence staining and tris-color flow cytometry in vitro.Results Compared with the ratios of CD4+/CD8+(1.57±0.04) in healthy people,the ratios of CD4+/CD8+(1.95±0.05) in RA in creased obviously (P＜0.05).Compared with the ratios of CD3+CD4+CD30-/CD3+CD4+CD30+(15.74±5.36) and CD3+CD8+CD30-/CD3+CD8+CD30+(15.82±4.03) inhealthy people,theratiosofCD3+CD4+CD30-/CD3+CD4+CD30+(20.72±7.34)and CD3+CD8+CD30-/CD3+CD8+CD30+ (19.81±3.28).FCM analysis showed that average percentages of CD4+(47.42%±2.68%) in RA increased sharply (P＜0.01) versus 40.27%±2.53% in healthy people,while the average percentages of CD8+,CD3+CD4+CD30+,CD3+CD8+CD30- and CD3+CD8+CD30+ did not changed versus healthy people.Conclusion The ratios of CD4+/CD8+,CD3+CD4+CD30-/CD3+CD4+CD30+ and CD3+CD8+CD30-/CD3+CD8+CD30+ in peripheral blood T cells were increased obviously in RA.It suggested that cellular immunity in RA is shifted to Th1 type immunity. 　　Key words：Rheumatoid arthritis(RA)；T cell subsets；Trisclolr flow cytometr