褪黑素对脊髓缺血－再灌注损伤抗氧化作用.docVIP

褪黑素对脊髓缺血－再灌注损伤抗氧化作用 　【关键词】 脊髓；缺血－再灌注；褪黑素；脂质过氧化 　　［摘要］ 目的：观察褪黑素对脊髓缺血－再灌注损伤的保护作用。方法：SD大鼠随机分为3组，一组仅行剖腹术，不钳夹动脉。另二组行缺血－再灌注术。分别在再灌注前后各5 min腹腔注射褪黑素和含2％乙醇的生理盐水。4 h后取腰骶段脊髓，测量组织中的丙二醛、过氧化氢酶和谷胱甘肽过氧化物酶。结果：再灌注后4 h，对照组组织中丙二醛显著升高，过氧化物酶和谷胱甘肽过氧化物酶活性降低，褪黑素组与对照组相比组织中丙二醛水平明显降低，而过氧化氢酶和谷胱甘肽过氧化物酶活性增加。结论：运用外源性褪黑素能明显减轻脊髓缺血－再灌注后的氧化损伤。 　　［关键词］ 脊髓；缺血－再灌注；褪黑素；脂质过氧化 　　Protective Effect of Melatonin on Experimental Spinal Cord IschemiaReperfusion Injury 　　Abstract：Objective To observe the protective of melatonin on experimental spial cordischemia－reperfusion injury.Methods Male Spryue－Dwleys rats were divided into three groups,rats inone group only had laparotomy without aortic occlusion;in other two groups had their infrarenal arota temporarily occluded for 30 min,then were given either a 20 mg/kg single dose of melatonin and saline containing 2% ethanol intraperitoneally twice,5 min fefore aortic occlusion and 5 min after the clamp was removed.At 4hours after reperfusion,the animals were authanized and lumboscaral spinal cord tissue was removed for the determination tissular malondialdehyde(MDA) levels and relevant enzyme activities.Results Malondialdedhyde levels,indicating the shemoperated group.Catalase (CAT) and glutathione peroxidase(GSHPx) enzyme activities were decreased in the the nonmelatonin treated group compared to the shamoperated group.Melatonin can attenuate the changes(Plt;0.05).Conclusion Melatonin can effectively protected spinal cord from ischemiareperfuion injury. 　　Key words：Spinal cord; Ischemiareperfusion; Melatonin; Lipid peroxidation 　　各种原因引起的脊髓压迫及胸腹主动脉外科手术中主要根动脉的阻断、损伤均可造成脊髓缺血性损伤(spinal cord ischemic injury)，导致神经功能障碍。研究已证实，脊髓缺血后再灌注过程中可进一步加重神经元损害，造成脊髓缺血再灌注损伤，最终导致肢体神经功能进一步加重。中性粒细胞释放的氧自由基可损伤细胞膜［1］，自由基诱导的脂质过氧化是脊髓损伤致残的主要原因之一［2］。褪黑素(melatonin,MT)主要是由松果体分泌的激素，能直接清除自由基，减轻细胞的过氧化损害。本文通过生化指标的观察，了解MT对脊髓缺血再灌注损伤的保护作用。 　　1　材料与方法 　　1．1　实验动物分组及药物准备　　　　　　　 　　健康封闭群SD雄性大鼠30只，体重220 g~250 g，随机分为3组，即：空白组，手术操作同其他组，不阻断血流。MT处理组，再灌注前后5 min腹腔注射褪黑素(20 mg／kg)各一次。对照组，持续缺血30 min，再灌注4 h。再灌注前后5 min腹腔注射即予