急性心肌梗塞患者血清miR-208a检测及其临床意义.docVIP

急性心肌梗塞患者血清miR-208a检测及其临床意义目的 探讨检测miR-208a在急性心肌梗塞(AMI)患者早期血清中的表达及其临床意义。方法 采用实时荧光定量RT-PCR(Real-time RT- PCR) 检测我院收治的35例AMI患者发病后不同时间与30例正常对照组人员血清miR-208a表达水平，并与血清肌酸激酶同工酶(CK-MB)和心肌肌钙蛋白I(CTn1)水平进行对比分析。结果 正常人血清miR-208a维持在相对较低的水平。AMI发作后1h患者血清miR-208a的表达水平可明显升高(P0.05)， AMI发作后3h血清miR-208a即可达到峰值。血清miR- 208a的表达与CK-MB和CTn1呈正相关(P 0.01)。 结论 AMI患者早期血清miR-208a表达显著升高，可能是潜在的AMI早期血清标记物。 心肌梗塞；miR-208a；血清；标记物Detection of miR-208a in Serum of Patients with Acute Myocardial Injury and its Clinical Significance JIA Ting-jie1, DANG Jung-qiang2, (1 Department of internal medicine, Lanzhou University Hospital, Lanzhou 730000, China; 2 Department of general surgery, The hospital affliated to Lanzhou Military Region Air Force, Lanzhou, 730000, China ) [Abstract] Objective To investigates the expression of miR-208a in serum of patients with acute myocardial injury (AMI), as well as their clinical significance. Method The blood samples were collected from 42 AMI patients at different times and 30 normal subjects．The expression levels of miR-208a in the serum were measured by reverse transcription polymerase chain reaction. The relationships between miR-208a and CK-MB, CTn1 were analyzed．Result Expression level of miR-208a in AMI patients′ serum, which was very low under normal condition, was significantly increased at 1 h after AMI. The levels of miR-208a could been found reaching peak values at 3 h post AMI. Pearson’s correlation analysis also showed that the level of serum miR-208a had positive correlation with CK-MB and CTn1 (P 0.01). Conclusion The significantly up-regulated expression levels of serum miR- 208a in AMI patients reflected the situation of myocardial Injury, indicating that miR- 208a can be used as a potential biomarker for AMI. [Key words] Acute myocardial infarction；miR-208a；serum； biomarker 急性心肌梗塞(AMI)是指冠状动脉急性闭塞，出现持久而严重的心肌缺血所致的局部心肌急性坏死。早期诊断AMI，准确地评价其病情严重度和判断预后，及时有效的干预治疗，是治疗AMI的关键[1]。目前许多AMI诊断标志物已被证实并应用于临床，但这些传统的标记物尚存在如诊断特异性差、早期诊断灵敏度不高、诊断窗口时间短等不足之处。miRNA是近年来发现的一类广泛存在于真核生物