1、3、4-三磷酸肌醇 56激酶基因的表征是水稻干旱和盐胁迫反应中必不可少的.pdfVIP

Plant Mol Biol (2011) 77:547–563 DOI 10.1007/s11103-011-9830-9 Characterization of an inositol 1,3,4-trisphosphate 5/6-kinase gene that is essential for drought and salt stress responses in rice Hao Du • Linhong Liu • Lei You • Mei Yang • Yubing He • Xianghua Li • Lizhong Xiong Received: 20 May 2011 / Accepted: 12 September 2011 / Published online: 25 October 2011 Springer Science+Business Media B.V. 2011 Abstract Drought and salt stresses are major limiting analysis of OsITPK genes revealed that they had different factors for crop production. To identify critical genes for tempo-spatial expression patterns, and the responses of stress resistance in rice (Oryza sativa L.), we screened DSM3 to abiotic stresses, including drought, salinity, cold, T-DNA mutants and identified a drought- and salt-hyper- and high temperature, were distinct from the other five sensitive mutant dsm3. The mutant phenotype was caused members in rice. These results together suggest that DSM3/ by a T-DNA insertion in a gene encoding a putative inositol OsITPK2 is an important member of the OsITPK family for 1,3,4-trisphosphate 5/6-kinase previously named OsITPK2 stress responses, and an optimal expression level is essential with unknown function. Under drought stress conditions, for drought and salt tolerance in rice. the mutant had significantly less accumulation of osmolytes such as proline and soluble sugar and showed significantly Keywords Abiotic stress Inositol phosphate Oryza reduced root volume, spikelet fertility, biomass, and grain IP3 Secondary signaling yield; however, malondialdehyde level was increased in the mutant. Interestingly, overexpression of DSM3 (OsITPK2) in rice resulted in drought- and salt-hypersensitive pheno- Introduction