诊断畜禽寄生虫病的一种新技术(A new technique for diagnosis of parasitic diseases of livestock and poultry).docVIP
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诊断畜禽寄生虫病的一种新技术(A new technique for diagnosis of parasitic diseases of livestock and poultry)
诊断畜禽寄生虫病的一种新技术(A new technique for diagnosis of parasitic diseases of livestock and poultry)
A new technique for diagnosis of parasitic diseases of livestock and poultry
[keyword] parasitic diseases
Over the years, we studied a method for qualitative and quantitative detection of parasitic disease of new technologies, including new egg counter (patented), operating procedures, eggs per gram of feces number calculation formula, the dilution volume and is stool sampling volume, liquid stool sedimentation and the feasibility of (i.e. in the determination of distribution to uniform distribution of liquid manure precipitation of the bdelloid giant kiss spine head Fasciola eggs, egg uniformity) were studied, and the clinical test results show that this technology is used for qualitative and quantitative detection of parasitic disease diagnosis is reliable.
1 manufacture of new egg counter
In a 2mm thick slide draw 2 1cm 2 boxes in each box, every 1mm draw 1 vertical lines (to avoid duplication and omission of eggs, then count) with 3 1mm thick glass strips are respectively stuck in the middle of the two squares and on both sides, then another 1mm thick glass stick in the glass, the formation of two is a counting chamber, a counting chamber volume of 0.1mL, shown in figure 1.
2 other appliances
Microscope, (or other small scale balance weigher), cylinder, container (beaker, plastic cup or iron cup can), copper mesh, pipette.
3 operation procedure
3.1 quantitative weighing the feces of the tested livestock (when detecting Fasciola disease, at least the sheep feces 4.7G, cow dung 27.5g), with quantitative stool 3~5 times the body water into a paste.
3.2 filter with copper screen and filter with proper water edge.
3.3 the filtrate precipitation of 30min (precipitation time detection of Fasciola eggs such as large eggs, Paramphistomum eggs, eggs and other spine head is 15 ~ 20min precipitation time, detection of oocysts such as 1H); the supernatant was removed, then water prec
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