连续酶反应器（Continuous enzyme reactor）.docVIP

连续酶反应器（Continuous enzyme reactor） Lipase catalyzed synthesis in a continuous enzyme reactor Study on maltose laurate Gao Xu, Liu Qiaoyu, Zhang Xiaoming, Feng Biao (Key Laboratory of food science and safety, Ministry of education, Jiangnan University, Jiangsu, Wuxi 214122) Abstract: maltose laurate was synthesized by continuous enzyme reactor (CSTR) and immobilized lipase. Continuous production was determined The optimum conditions for the process are: in acetone medium, the concentration of maltose in the starting reactor is 50 mmol/ L, and the lauric acid concentration is 200 Mmol/ L, with an enzyme dosage of 10 g, added 4 g maltose per day, 200 mmol/ L lauric acid acetone solution, with 0115 mL/ min Inlet reactor. The maltose ester yield of this reactor can reach 12132 g/ (L = D), and the conversion rate of maltose is 58%, which is higher than that of the maltose Intermittent production, and the addition of molecular sieves is unnecessary in production. The method of separation and purification of product was studied The purity of maltose lauryl ester obtained by 3 times washing and centrifugal treatment was 94.16%. Keywords: continuous enzyme reactor; immobilized lipase; maltose laurate Sugar esters are a group of renewable resources, non-toxic, Yi Sheng The material is degraded by non-ionic surfactant and can be controlled by The composition of fatty acids and sugars, and the degree of acylation, are different Product of HLB values. Although studies on maltose esters have been reported Not many, but it has a wide range of applications in food, cosmetics and other fields Potential [1-2]. Currently, the market for sugar ester products is essentially chemical synthesis The product is a complex mixture that requires a high purity product Make a complicated separation. Enzyme catalyzed synthesis of sugar esters is reversed It should be high in selectivity, mild in reaction conditions, less in by-products and easy in product Separation and purification. At present, th