血红素氧合酶-1抗炎效应对大鼠复苏后心功能的影响-第三军医大学学报.DOCVIP

血晶素对大鼠复苏后心肌损伤的保护作用和机制 采用窒息法制作大鼠心跳骤停心肺复苏（CPR）模型。实验动物分为4组：假手术组、CPR组、Hemin组（血晶素组）、Hemin+ZnPP组（血晶素+锌原卟啉组）。假手术组术后6、24 h 2个观察时相点，每时点6只鼠，各复苏组复苏后6、24 h 2个观察时相点，每时相点8只鼠。各组蛋白免疫印迹法检测心肌组织HO-1蛋白水平；全自动生化分析仪测定血清肌酸肌酶同功酶（CK-MB）、乳酸脱氢酶（LDH）含量；ELISA法测定大鼠血清TNF-α、IL-6浓度；观察心肌超微结构变化。结果 1、与假手术组比较，CPR组、Hemin组、Hemin+ZnPP组复苏后6 h及24 h HO-1蛋白水平、CK-MB和LDH活性显著增高（P＜0.05））））））The anti-inflammatory effect of heme oxygenase-1 on postresuscitation myocardial injury in rats Wang Xiaohong, Zhou Wenjie, Yang Xiaojun, Fang Xiangshao, Huang Zitong (Department of Intensive Care Unit, General Hospital of NingXia Medical University, Yinchuan, 750004, China 【Abstract】Objective To explore the anti-inflammatory effects of heme oxygenase-1 on postresuscitation myocardial injury in rats. Methods Male Sprague-Dawley rats were asphyxiated for 9 minutes and resuscitated, rats were randomly divided into 4 groups: Sham operated group, CPR group, Hemin group, Hemin+ZnPP(zinc protoporyphyrinIX) group . Resuscitated groups have two observation points: 6 hours and 24 hours after cardiopulmonary resuscitation (n=8 for each time point), Sham operated group 12 rats, divided in two observation points, according to 6hours or 24hours after surgery, each comprising 6 rats. HO-1 expression in cardiac tissue was detected by Western blot. Serum creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) levels were detected by automatic biochemistry analyzer. Serum concentrations of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) were determined by enzyme-linked immunosorbent assay (ELISA). Cardiac ultrastructure was examined by transmission electron microscopy. Results 1. Compared with sham operated group, the HO-1 expression、CK-MB 、LDH levels were significantly increased at 6hours and 24hours after resuscitation in the CPR group, Hemin group and Hemin+ZnPP group （all P0.05）. The levels of TNF-α in CPR group and Hemin+ZnPP group were significantly increased at 6hours and 24hours after resuscitation , but in Hemin group