纳米液态氟碳脂质微球的制备及体外聚集试验研究-第三军医大学学报.docVIP

纳米液态氟碳脂质微球超声造影剂的制备及体外聚集实验研究 陈 松，康 娟，王志刚，李 攀，伍 星，许川山 （重庆医科大学附属第二医院超声影像学研究所，重庆400010） 摘 要： 目的 制备一种新型超声造影剂－纳米液态氟碳脂质微球造影剂，并研究其体外基本特性及聚集后增强超声显影性能。方法 采用高压均质法制备纳米液态氟碳脂质微球超声造影剂，检测一般理化性质，再制备生物素化微球，以空白微球为对照组，分别在加入亲和素前后，观察体外聚集情况及超声显影效果，并用DFY超声图像定量分析统计软件计算、比较超声图像平均灰度值。结果 所制备的液态氟碳脂质微球外观呈乳白色的混悬液，镜下微球形态圆整，大小均一，性质稳定，粒径（171.9±91.0）nm；制备的生物素化微球在加入亲和素后聚集成团状，并可增强超声显影，经DFY软件分析，加入亲和素前后超声图像平均灰度值差异有统计学意义。结论 成功制备出稳定的纳米液态氟碳脂质超声造影剂，并采用生物素亲和素法证明了该微球具有聚集后增强超声显影性能。 关键词： 纳米；液态氟碳；超声造影剂 中图法分类号： 文献标识码：A Preparation and aggregation study of a novel perfluorocarbon lipid microlipheres ultrasound contrast agent CHEN Song, KANG Juan，WANG Zhi-gang, LI Pan, WU Xing, XU Chuan-shan （Institute of Ultrasound Imaging, Chongqing University of Medical Sciences,Chongqing 400010，China） Abstract：Objective To develop a novel nanoscale ultrasonic contrast agent— perfluorocarbon lipid microlipheres and to investigate their basic charactors and capability to enhance ultrasound images when aggregated . Methods Perfluorocarbon lipid microlipheres were prepared by high pressure homogen. The physico-chemical properties of the microlipheres was detected. Biotinylated perfluorocarbon lipid microlipheres were prepared for ultrasound imaging before and after adding stepavidin. The average gray scale value of the captured ultrasound images were analyzed by the DFY software. Results These microlipheres were uniform and stabilized, and the mean size is （171.9±91.0）nm. There was no visuable ultrasound signals scattered back from the microlipheres before adding strepavidin, while obviously ultrasound echo signals were observed after adding strepavidin. There was significant difference between ultrasound images before/after adding strepavidin, which were analyzed by DFY software. Conclusions A steady perfluorocarbon lipid microlipheres were prepared and the biotinylated microspheres could aggregate together in the existence of strepavidin and enhance the ultrasound image. The aggregated microlipheres might be taken