PE-Operetta介绍.pdfVIP

Operetta 高内涵筛选系统 刘晓娟 HCS产品专员 2012-11月 11 HCS 的概念 Operetta高内涵成像系统（High Content Imaging System）是一个成熟的结合荧光显微 成像及多参数定量图像分析的技术。这种强大的组合为研究人员提供了快速有效 的工具，使其能够迅速获得目标蛋白或化合物在细胞中的作用，有效加快了您的 决策时间并提高了工作效率。 22 20x long WD objective, HeLa cells stained with Alexa Fluor®488 labeled anti tubulin antibody and DRAQ5 33 Mouse embryonic stem cell colonies after 3 days of culturing Early mouse embryonic stem cell colonies growing on a imaged on Operetta fibroblast feeder layer imaged on Operetta 44 Yeast 100x Objective 55 Brightfield #2 c. elegans brightfield 66 eGFP #2 c. elegans heatshock induced GFP 77 Zebrafish Brightfield GFP – Vascular Structure dsRED – Red Blood Cells 88 Immunohistochemistry (IHC) 99 Small Organism Imaging 1010 Whole Organism Imaging Zebrafish embryo, 4 days post fertilization C. elegans DIC Gives higher resolution and optical sections, But generally not useful for plastic substrates 1111 Operetta 硬件配置 Xenon lamp 专门为HCS设计的光路 Transmission light