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平板计数法-计数原则
STANDARD PLATE COUNT, COLIFORM, AND SIMPLIFIED COUNT METHODS
[Unless otherwise stated all tolerances are ±5%]
SAMPLES
1. Laboratory sample requirements (see CP item 33 34) ________
STANDARD PLATE AND COLIFORM METHODS
DILUTING SAMPLES
2. Work Area ________
a. Level plating bench not in direct sunlight ________
b. Sanitized immediately before start of plating ________
3. Selecting Dilutions ________
a. Standard Plate Count ________
1. Plate two decimal dilutions per sample ________
2. Select dilutions to yield one plate with
25-250 colonies ________
a. Raw milk is normally diluted to 1:100 and
1:1000 ________
b. Finished products are normally diluted to
1:10 and 1:100 ________
c. The above are general guidelines and may have
to be adjusted on a case by case basis
(dilutions below 1:10 not required) ________
b. Coliform Counts ________
1. For milk samples, 1 mL direct and/or decimal
dilutions ________
2. For all other products, distribute 10 mL of a
1:10 dilution among three plates, generally high
fat and viscous products ________
4. Identifying Plates
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