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大鼠白介素1β (il-1β)酶联免疫分析试剂盒使用说明书(ELISA kit for rat interleukin 1 beta (IL-1 beta))
大鼠白介素1β (il-1β)酶联免疫分析试剂盒使用说明书(ELISA kit for rat interleukin 1 beta (IL-1 beta))
ELISA kit for rat interleukin 1 beta (IL-1 beta)
This kit is for research use only
Test range: 3.12 pg/ml - 200 pg/ml
Minimum detection limit: 0.78 pg/ml
Specificity: this kit allows simultaneous detection of natural or recombinant rat IL-1 beta and has no cross reactivity with other related proteins.
Validity: 6 months
Prospective application: ELISA method for quantitative determination of IL-1, beta in serum, plasma, cell culture supernatant or other related biological fluids in rats.
Explain
1. kit kept: -20 degrees (when not longer); 2-8 degrees (frequently used).
2., concentrated liquid wash at low temperature will be salted out, diluted in the water bath heating, help dissolve.
3., the English and Chinese manual may be out of order. Please use the English manual.
4. the newly opened enzyme plate hole may contain a small amount of water samples, this is normal, and will not have any effect on the results of the experiment.
Summary
Interleukin -1 (IL-1), also known as lymphocyte stimulating factor, is divided into two kinds: IL-1 alpha and IL-1 beta. They are expressed by different genes, the molecular weight is 15kDa, but the PI is 5 and 7 respectively. Both were composed of 159 and 153 amino acids, with only 26% homology between amino acid sequences, but were combined with the same membrane receptor. IL-1 is produced mainly by monocytes and macrophages in the blood. T lymphocytes, B lymphocytes, various epithelial cells, endothelial cells and stromal cells also produce IL-1. IL-1 in the blood is mainly IL-1 beta produced by monocytes and macrophages. IL-1 is a major regulator of acute immune response and plays a fundamental role in the activation of T cells and the induction of IL-2 production. These regulatory actions are produced mainly by the action of the bone marrow stem cells in the nervous system. Most of these regulatory effects are directly produced by IL-1 beta, but some
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