DNJ对正常小鼠脂代谢的影响及作用机理初探-现代食品科技.PDFVIP

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.4 DNJ对正常小鼠脂代谢的影响及作用机理初探 1 1 1 2 王玲 ，曾艺涛 ，丁晓雯 ，黄先智 （1.西南大学食品科学学院，重庆 400716）（2.家蚕基因组生物学国家重点实验室，重庆 400716） 摘要：本文为探索 DNJ 对脂代谢的影响及作用机理，给正常小鼠灌胃 DNJ 40 d 后，用试剂盒测定各实验动物脂代谢相关生化指 标、酶活及抗氧化指标。结果显示与阴性对照组比较，8.0 mg/(kg bw⋅d)剂量 DNJ 使雌、雄小鼠的体重增加率分别降低 59.40%和 9.15%； 腹腔脂肪系数降低 38.30%和 15.18%；肝脏脂肪含量降低 18.42%和 36.66%；血清 TC 降低27.40%和 28.57% ，TG 降低0.89%和 15.03%， HDL 增加 42.19%和 6.80%；肝脏 SOD 活性上升29.24%和 25.17% ，GSH 增加 16.29%和 15.00%，8-isoprostane 下降 18.53%和 15.19%； 雌鼠 FAS、ACO 活性下降 14.02%和 9.67%，雄鼠ACO 活性上升21.63%。说明适量DNJ 主要通过抑制雌鼠脂肪酸合成、加速雄鼠脂 肪酸氧化来减少脂肪蓄积，且对雌鼠具有更好的预防肥胖作用；同时可增强小鼠机体抗氧化能力，预防脂代谢异常。 关键词：脂代谢；酶活；抗氧化 文章篇号：1673-9078(2017)4-6-12 DOI: 10.13982/j.mfst.1673-9078.2017.4.002 Effect of 1-Deoxynojirimycin on Lipid Metabolism in Normal Mice and Preliminary Exploration of the Underlying Mechanism 1 1 1 2 WANG Ling , ZENG Yi-tao , DING Xiao-wen , HUANG Xian-zhi (1.College of Food Science, Southwest University, Chongqing 400716, China) (2.State Key Lab of Silkworm Genome Biology, Chongqing 400716, China) Abstract: To explore the effect of 1-deoxynojirimycin (DNJ) on the lipid metabolism, and the corresponding mechanism, normal mice were fed DNJ by gavage for 40 days, and then biochemical indicators of lipid metabolism, the activity of enzymes, and the antioxidant indices of the animals were determined using enzyme-linked immunosorbent assay (ELISA) kits. Compared with the negative co