催化荧光法测定痕量铜的探究性试验设计与实践-大学化学.PDFVIP

大学化学(Daxue Huaxue) 58 Univ. Chem. 2017, 32 (1), 58－61 January ∙化学实验∙ doi: 10.3866/PKU.DXHX201609031 催化荧光法测定痕量铜的探究性实验设计与实践 盛 丽* 赵志惠 米 莹 苏碧泉 荆王莉 陶彩虹 (兰州交通大学化学与生物工程学院，兰州730070) 摘要：基于铜(II)对高碘酸钾氧化番红花红T 具有催化作用，建立催化动力学荧光法测定痕量铜的新体 系。体系在加入铜(II)后，在580 nm 波长处的荧光强度明显降低，且在2.50 －32.50 ng ∙mL－1 范围内 铜(II)的浓度与体系的lg(F /F)呈线性关系。基于催化动力学荧光分析法测定痕量铜的新方法实验，探讨 0 了仪器分析实验课探究性实验的设计与实施方法。同时，为实现探究性实验教学，教师要严把命题关和 可行性分析关，以期达到探究性实验设计的教学效果。 关键词：仪器分析实验；探究性实验设计；铜；催化动力学荧光法 中图分类号：G64 ；O657 Measurement of Tracing Copper Using Catalytic Kinetic Fluorescence Method: Design and Practice of an Inquiry Experiment SHENG Li* ZHAO Zhi-Hui MI Ying SU Bi-Quan JING Wang-Li TAO Cai-Hong (School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, P. R. China) Abstract: Based on the catalytic effects of Cu(II) on KIO oxidized safranine T, a new system for determining 4 tracing copper with catalytic kinetic fluorescence method is established. After adding Cu(II), the fluorescence intensity at 580 nm is significantly reduced. Within the concentration range of 2.50－32.50 ng ∙mL－1, a linear relationship between the concentration of Cu(II) and lg(F /F) in the system is observed. Based on this new 0 experiment, the design and implementation of instrumental analysis laboratory is investigated. Meanwhile, in order to achieve high quality of the inquiry experiment teaching, teachers should be responsible for pr