哺乳动物胚胎透明带脱落机制的研究进展.PDFVIP

5 3 ( ) 生命科学研究 Vol . 5 No . 3( Suppl. ) 2 0 0 111 Life Science Research Nov. 2001 X 后晓南, 王智彪 ( , 400016) : 胚胎着床是一个连续的动态过程, 其中胚泡从透 带中准时孵出是着床的关键. 透 带脱落的机制主 要是子宫或( 和) 胚泡分泌物部分或全部溶解透 带后, 胚泡在细胞数量增加及细胞运动的机械压力作用下通 过透 带的某 一位点孵出. : 胚胎 透 带 孵化 机制 : R321. 3 : A : 1007 - 7 847( 2001) S0 - 0198- 05 The Mechanism of Mammalian Embryo Hatching HOU Xiao-nan, WANG Zh-i biao (I nstitute of Ultrasound Engineering in Medicine , Chongqing Medical University , Chongqing 400016, China) Abstract:Su ccessful implantation in mammals requ ires hatching of the blastocyst from the zona pellucida. But the mechanism of mammalian embryo hatching is still not clear : Some data show that successful hatching in vitro is de- pendent on a sufficiently high number of embryonic cells, which enables blastocyst expansion and zona shedding. In vivo, the low er number of embryonic cells detected in zona-free blastocysts indicates that the underlying mechanism of zona escape is different, it does not depend on blastocyst expansion, and presumably involves lytic factors from the uterus. Results of experiments suggest that a proteinase called / strypsin0 is directly involved in hatching of iso- lated mouse blastocysts in vitro . Strypsin is a trypsin- like proteinase, and it is present in mouse blastocyst and ex- hibits certain properties characterist ic of membrane- associated enzymes. These results suggest that not only tension but also lytic enzymes are requ ired to rupture from zona pellucida. Key word: embryo zona pellu cida hatching mechanism ( L