地黄属核基因与叶绿体基因DNA条形码比较研究.PDFVIP

中草药 Chinese Traditional and Herbal Drugs 第 48 卷 第 1 期 2017 年 1 月 • 165 • 地黄属核基因与叶绿体基因 DNA 条形码比较研究 李建芳，黄 静，程芳婷，杜 优，刘占林* 西北大学生命科学学院，西部资源生物与现代生物技术教育部重点实验室，陕西 西安 710069 摘 要：目的 比较单拷贝核基因、叶绿体基因及ITS 等 DNA 条形码在地黄属的物种分辨率，探讨地黄属种间亲缘关系。 方法 利用距离法与建树法分析了地黄属植物 13 个居群的 12 个单拷贝核基因、6 个叶绿体基因以及 ITS 片段物种鉴定成功 率，构建了地黄属系统发育树。结果 叶绿体matk 和 rbcL 、单拷贝核基因物种分辨率普遍较低，多基因联合分析有助于物 种鉴定成功率的提高。在 NJ 树中，地黄-茄叶地黄聚为一支，其他物种都是单系群，裂叶地黄位于系统发育树基部。结论 ITS 是地黄属物种鉴定的核心条形码，psbA-trnH 、trnL-F 、trnM-V 、trnS-G 可以作为备选方案，核基因 RR255 与 R257 可用作地 黄专用鉴定条形码。四倍体地黄起源方式有待进一步澄清。 关键词：地黄属；DNA 条形码；核基因；叶绿体基因；亲缘关系 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2017)01 - 0165 - 07 DOI: 10.7501/j.issn.0253-2670.2017.01.023 Comparison on DNA barcodes of nuclear and chloroplast gene fragments in Rehmannia Libosch. ex Fisch. et Mey. (Rehmanniaceae) LI Jian-fang, HUANG Jing, CHENG Fang-ting, DU You, LIU Zhan-lin Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Sciences, Northwest University, Xi’an 710069, China Abstract: Objective To compare the usage of single-copy nuclear genes (SCNGs), chloroplast gene fragments and internal transcribed spacer (ITS) as DNA barcodes in plants of Rehmannia Libosch. ex Fisch. et Mey. and elucidate the interspecific relationships in the genus. Methods Distance and tree-based methods were performed to analyze 12 SCNGs, six cpDNA regions, and ITS fragment in 13 populations of Rehmannia Libosch. ex Fisch. et Mey. Neighbor-joining tree was constructed to investigate the relationships among the species. Results CpDNA regions (matK and rbcL) and SCNGs had lower identification rates than others, especially ITS. Multiple DNA barcodes combination would be helpful to improve the species identification rate. The NJ tree indicated that R. glutinosa and R. solanifolia were clustered together, other species in the genus were monophyleti