17-第五章 培养基及设备的灭菌（17- chapter fifth sterilization of culture medium and equipment）.docVIP

17-第五章 培养基及设备的灭菌（17- chapter fifth sterilization of culture medium and equipment） The fifth chapter: sterilization of culture medium and equipment First, the purpose, requirements and methods of sterilization of culture medium I. definitions 1. Definition of medium sterilization Bacteria or spores that kill living bacteria from a culture medium or remove them from them. Sterilization of industrial scale liquid cultures is more common than elimination of miscellaneous bacteria. 2, the difference between sterilization and disinfection Sterilization: the removal or removal of all microorganisms in the environment, including vegetative cells, bacteria, spores, and spores, by physical or chemical methods Disinfection: the killing of pathogens in containers, containers, or containers by physical or chemical methods. Two. The purpose of culture medium sterilization 1, in the fermentation process with other miscellaneous bacteria caused by the consequences: Production of bacteria and other bacteria at the same time, the production of bacteria loss of production capacity; In the process of continuous fermentation, the growth rate of the hybrid bacteria is sometimes faster than that of the producing bacteria, and the result is that the fermentation tank is dominated by miscellaneous bacteria; The bacteria and their substances make it difficult to extract and refine them Microbes degrade target products; The bacteria contaminate the end product and contaminate the final product; During the fermentation of such contaminated phage can make the producing bacteria lysis. 2, industrial specific measures include: (1) the culture medium and equipment used must be sterilized; (2) the air used in aerobic culture should be sterilized; (3) the equipment should be tight and the fermentation tank maintains positive pressure; (4) the materials added to the culture process shall be sterilized; (5) the use of pollution-free pure seeds. 3, the purpose of sterilization of culture medium Kill mi