改良焦性没食子酸法培养厌氧菌（Improved pyrogallic acid method of anaerobic bacteria culture）.docVIP

改良焦性没食子酸法培养厌氧菌（Improved pyrogallic acid method of anaerobic bacteria culture） Improved pyrogallic acid method of anaerobic bacteria culture Yuan Guixiu... Lin... Morning... (Department of Microbiology and immunology, School of medicine, Jinan University, Guangdong, Guangzhou 510632) Keywords:.. pyrogallil acid.; anaerobic bacteria; culture medium. CLC number:.. R378.... Document identification code:.. A.... Article number:.. 1000-9965 (1999) 04- 0024- 02 At present.. anaerobic bacteria culture method mainly pyrogallic acid method, anaerobic method, anaerobic tank and anaerobic culturing box method [1]. Bore and dislike The incubator needs some equipment or special equipment, so its application is limited. Pyrogallic acid method or anaerobic bacteria are anaerobic method Because of its simplicity and easy operation, isolation and culture are still widely used in microbiology teaching or primary medical care units in universities and colleges, but the separation effect is not easy Control. Therefore, the laboratory in the teaching practice, cultivation of anaerobic bacteria by improved pyrogallic acid separation, colony and hemolysis separated better In ordinary pyrogallil acid or anaerobic method. Now summarized as follows. 1. Materials and methods (1): ordinary pyrogallil acid anaerobic bacteria to blood agar plate, the outer side of the central plate cover of about 4 cm in diameter circle The two layer shaped gauze, put 0..2 g pyrogallic acid and then cover the two layer gauze of equal size, and then add the mass fraction of 10%NaOH 0..5 mL. Speed the flat dish upside down on it, the plate around with melted paraffin closed, put 37.. culture 24~, 48 h after the observation. (2) improved pyrogallic acid method: on the basis of the common methods, in the preparation and training with the composition of chemical modified blood agar Well, one is made of filter paper, the bag contains a proper amount of carbon residue instead of gauze or cotton; two is using anhydrous s