甲胎蛋白基因甲基化模式对基因表达地影响.pdf

甲胎蛋白基因甲基化模式对基因表达地影响.pdf

英文摘要 3 ofDNAand treatmentofDNA:。Ihe Preparation blsulphate with DNAwas ClassicGenomicDNA genomic prepared inTEbuf.ferat4℃tilluse.ARer Is01ation stored Kit,and of DNAwith digestiongehomic usedto bisulfite、vas modi矽genomicDNA行agment.The werecleaned WizardDNA ÷ productS upusingPromega Purificationto offexcessSodium Systemget bisulfite,then, DNA toobtain DNA. oVemight MOdificatiVe precipitated ModiflcativeDNA was storeat一20℃till sample6nally 6Jrtheruse. 4To the islandofAFP was CpG gene designprimers:The identifiedwithanassistanceofthe islandidentification CpG //Ⅵr、^r、^r.ebi.ac.uk/em sofeware(http: boss/cpgplot/index. forMSPandBSP were html).Theprimerpairs analysis with sofeware / designedtheprimerdesign (http: /micro—gen.ouhsc.edu/cgi-bin/primer3www.cgi). 5 chain method reacti01:l Methylationspeci丘cpolymerase the oftwoareainthe (MSP)analyzedmethylationdensity humanAFP ofdifferent theMoDNAas gene cells:Using AFP MSP template,amplmcatedgene仔agmentby primers and

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