英文摘要
3 ofDNAand treatmentofDNA:。Ihe
Preparation blsulphate
with
DNAwas ClassicGenomicDNA
genomic prepared
inTEbuf.ferat4℃tilluse.ARer
Is01ation stored
Kit,and
of DNAwith
digestiongehomic
usedto
bisulfite、vas
modi矽genomicDNA行agment.The
werecleaned WizardDNA ÷
productS upusingPromega
Purificationto offexcessSodium
Systemget bisulfite,then,
DNA toobtain DNA.
oVemight MOdificatiVe
precipitated
ModiflcativeDNA was storeat一20℃till
sample6nally
6Jrtheruse.
4To the islandofAFP was
CpG gene
designprimers:The
identifiedwithanassistanceofthe islandidentification
CpG
//Ⅵr、^r、^r.ebi.ac.uk/em
sofeware(http: boss/cpgplot/index.
forMSPandBSP were
html).Theprimerpairs analysis
with sofeware /
designedtheprimerdesign (http:
/micro—gen.ouhsc.edu/cgi-bin/primer3www.cgi).
5 chain method
reacti01:l
Methylationspeci丘cpolymerase
the oftwoareainthe
(MSP)analyzedmethylationdensity
humanAFP ofdifferent theMoDNAas
gene cells:Using
AFP MSP
template,amplmcatedgene仔agmentby primers
and
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