大鼠线栓法局灶性脑缺血再灌注模型的研究改进.docx

大鼠线栓法局灶性脑缺血再灌注模型的研究改进学生：方小逢1老师：叶小敏2 （1.咸宁学院药学院，咸宁，437100；2.武汉市食品药品检验所，武汉，000000）我的参考文献还没有整理好，你帮我看看正文里的内容有那些地方要改进，麻烦了！！摘　要:目的　改进大鼠线栓法局灶性脑缺血再灌注模型的制备方法，建立一种比较系统,操作简单,成功率高的大鼠大脑中动脉缺血(MACO)再灌注动物模型。方法　参照文献方法,将头端用石蜡处理过的尼龙钓鱼丝线,从左侧颈总动脉向颈内动脉插入,可逆性阻断大鼠一侧中动脉，制作短暂脑缺血模型，分别对模型大鼠进行神经行为学、四肢协调性检查；术后24h处死大鼠取脑组织进行四氮唑红（TTC）染色鉴定模型的成功与否。假手术组不进行动脉阻塞。结果　在模型制作过程中,遇到的最大困难是栓线插线时无法正确插入颈内动脉或者是插入过深。且不同体重的大鼠所需的栓线头大小不一，插入的深度也有明显的差别。除了改进栓线，方便标记栓线插入深度,直接在颈总、颈内动脉套上一根丝线牵拉,既有助于手术分离,又有助于控制出血;遇到栓线插入困难时,颈总、颈内动脉的复流可以有效地消除颈内动脉痉挛,成功率明显提高。结论　采用预先在颈总、颈内动脉套线,可以方便有效地控制出血。插线困难时,颈总、颈内动脉的复流可以提高成功率。改进后的模型成功率相对提高，制作良好的栓线、熟练的操作和体重合适的大鼠是制作模型成功的关键。　本方法能明显缩短手术时间,减轻对动物的损伤,且制备的模型可靠,脑缺血部位稳定。关键词:动物模型;SD 大鼠;线栓法;局灶性脑缺血/ 再灌注Modification of suture methods for rat model of focal cerebral ischemia reperfusionStudent：Fang Xiao Feng1Instructor：Ye Xiao Min 2 School of Pharmacy, Xianning College, Xianning,437100；2、The Food and Drug Inspection, WuHanChina, 000000)Abstract :Objective 　To probe the modification of suture methods for rat s model of focal cerebral ischemia reperfusion. To establish a model of rat ischemia reperfusion injury, in terms of the model, the operation will be simple, and the achievement ratio will be high.Methods 　According to the methods of literature , nylon thread with a paraffin in the end was inserted into the internal carotid artery ( ICA) in Sprague-Dawley rats to achieve an animal model of reversible middle cerebral artery occlusion.Stroke was induced by a 1.5h occlusion of the MCA using an intraluminal filament. Behavioral assessments were evaluated by neurological test, motor test. Brain tissue damage was detected in the ischemic animals 24h after the surgery by 2,3,5-triphenyl tetrazolium chloride(TTC) staining .Results 　While the model was made ,thebiggest difficulties is inserted correctly into the internal carotid artery ( ICA) or into a deep.And with different species and the weight of the rats ,the nylon thread is on all sizes, the insertion of detail are obvious difference. To reduce the bleedi