秋水仙素在烟苗染色体加倍试验中的重复利用研究.pdfVIP

湖南农业科学 2013 ，（17）：17~19 Hunan Agricultural Sciences 秋水仙素在烟苗染色体加倍试验中的重复利用研究 程 玲，冯 吉，余 君，蔡长春 （湖北省烟草科研所烟草生物技术工程研究中心，湖北 武汉 430030 ） 摘 要：为探讨秋水仙素在烟苗染色体加倍试验中重复利用的可行性，以白肋烟 F 代花药培养获得的单倍体无菌苗为材料，初始 1 浓度为 4 g/L 的秋水仙素溶液为加倍试剂，开展了 6 次重复利用的试验，测定死苗率、单倍体率、单双混倍体率、双单倍体率、 四倍体 率和双四混倍体率等染色体加倍指标 。 结果表明：从主要关注的双单倍体加倍效率来看，加倍效率呈现先上升后下降的变化趋势， 第 6 次重复利用后加倍效率为 21.3% ， 比第一次使用的加倍效率 22.8%仅下降 1.5 个百分点， 以第 3 次重复利用后的加倍效率最 高，为 36.1%。 关键词：烟草；染色体加倍；秋水仙素；重复利用 中图分类号：S572 文献标识码：A 文章编号：1006-060X（2013 ）17-0017-03 Repeated Utilization of Colchicines in Chromosome Doubling Experiment for Tobacco Seedling CHENG Ling, FENG Ji, YU Jun, CAI Chang-chun （Tobacco Biotechnology and Engineering Research Center, Hubei Institute of Tobacco Sciences, Wuhan 430030, PRC ） Abstract: In order to discuss the feasibility of repeated utilization of colchicines in chromosome doubling experiment for tobacco seedling, the haploid aseptic seedlings, cultivated from anthers of F1 generation of burley tobacco, were taken as materials and the colchicines solvent with initial concentration of 4 g/L was taken as doubling reagent to conduct six repeated utilization experiments, and the chromosome doubling indicators were determined, including dead seedling rate, haploid rate, haploid-diploid mixoploid rate, dihaploid rate, tetraploid rate and dihaploid-tetraploid mixoploid rate. The results showed that the doubling efficiency of dihaploid, which mainly be focused, showed a tendency of increasing fist and then decreasing; it was 21.3% after the sixth