卵形鲳鲹不同组织同工酶表达的差异.pdfVIP

第4卷第3期 南 方 水 产 VolI4．No．3 2 0 0 8年6月 South China Fisheries Science Jun．，2008 形鱼昌 不同组织同工 酶表达的差异 齐旭东 ，区又君 (1．广东海洋大学，广东湛江524025；2．中国水产科学研究院南海水产研究所，广东广州510300) 摘要：运用聚丙烯酰胺垂直梯度凝胶电泳法对卵形鲳够 (Trachinotus ovattt~)6组织 (肌肉、心脏、肝脏、肾脏、 脑、脾脏)的5种同工酶 (EST、LDH、MDH、ME和AST)进行了初步研究，并对5种同工酶的同工酶位点及 其酶谱表型进行了分析。结果表明，卵形鲳够的5种同工酶系统具有不同程度的组织特异性。EST由2个基因位 点编码，Est一1在这6种组织中都存在，Est一2只存在于肝脏和。肾脏中；LDH由3个基因位点编码，共检测到3条 谱带，Ldh-2只存在于肾脏中；MDH由2个基因位点编码，Mdh一1表达为MDH1和MDH2 2条酶带，Mdh-2表达 为MDH3 1条酶带，在肝脏中的活性最强；ME由1个基因位点编码，只检测到1条酶带，肝脏的含量丰富； AST由2个基因位点编码，只在肝脏和。肾脏中检测到，共检测到3条谱带。 关键词：卵形鲳够；同工酶；组织；表达 中图分类号：Q55 文献标识码：A 文章编号：1673—2227一(2008)03—0038一O5 Expression of five kinds of isozyme in different tissues of Trachinotus ovatus QI Xudong ’’，OU Youjun (1．Guangdong Ocean University，Zhanfiang 524025，China；2．South China Sea Fisheries Research Institute， Chinese Academy ofFishery Sciences，Guangzhou 510300，China) Abstract：Five isozymes(EST，ME，AST，LDH and MDH)from six tissues(muscle，heart，liver，kidney，brain and spleen)of Trachinotus ovattt~were preliminarily studied using vertical polyacrylamide gradient gel electrophoresis．The results showed that the tis— sue specificity of these isozymes is very obvious．The expression of enzyme gene loci and the activity of isozyme varied with different tis— sues．Th e number of EST isozyme bands was 3，which was encoded by 2 gene loci．Est一1 expressed in all 6 tissues，and Est-2 only ex— isted in liver and kidney．The number of LDH isozyme bands was 3，which was encoded by 3 gene loci．Ldh-2 only expressed in kid— ney．Two divisions of MDH were found from ovattt~．It was encoded