皮质酮致脊髓背角星形胶质细胞内钙浓度快速升高-第三军医大学学报.DOC

地塞米松快速升高大鼠脊髓背角星形胶质细胞钙浓度 曾俊伟，肖 智，陈远寿，田 虹，刘晓红*（遵义医学院基础医学院生理学教研室）观察( Dex)对培养的脊髓背角星形胶质细胞内Ca2 +浓度( [Ca2+]i )的影响作用机制培养纯化新生SD大鼠脊髓背角星形胶质细胞，激光共聚焦显微镜检测星形胶质细胞[Ca2+]i的变化。Dex可导致培养的脊髓背角星形胶质[Ca2+]i快速高，且呈现剂量依赖性；Dex诱导的星形胶质细胞[Ca2+]i升高是以外钙内流为主G蛋白阻断剂百日咳毒素100 ng/mL）可阻断Dex所致的星形胶质细胞[Ca2+]i升高效应而糖皮质激素胞浆可溶性受体拮抗剂RU38486(10μmol/L)Dex的效应蛋白合成抑制剂对Dex所致的星形胶质[Ca2+]i升高效应无Dex可能通过糖皮质激素膜受体非基因组快速高脊髓背角星形胶质细胞[Ca2+]i 。 关键词; 脊髓；星形胶质细胞; 钙Rapid elevation of calcium concentration in rat dorsal spinal cord astrocytes by dexamethasone Zeng Jun-wei, Xiao Zhi, Chen Yuan-shou, Tian Hong, Liu Xiao-hong* ( Department of Physiology, Zunyi Medical College, Zunyi 563000, China) [Abstract] Objective To explore whether dexamethasone (Dex) has an effect on the intracellular calcium concentration ([Ca2+]i) in cultured dorsal spinal cord astrocytes and the relative mechanism. Method Astrocytes from neonatal SD rat 基金项目: 国家自然科学基金 Supported by the National Natural Science Foundation of China 作者简介: ,女,人,士,主要从事痛觉方面研究。电话:(0852)8609442, E-mail: jw_zeng@ *通信作者: 刘晓红, 电话: (0852)8609442, E-mail: lxh680718@ spinal cord were ultured and purified. Changes of [Ca2+]i in cultured dorsal spinal cord astrocytes were detected with confocal laser scanning microscopy using ?uo-4/AM as a calcium ?uorescent indicator. Results Dex induced [Ca2+]i increase in cultured dorsal spinal cord astrocytes within several minutes. The rapid elevation of [Ca2+]i in astrocytes by Dex was concentration-dependent and could be blocked by pertussis toxin (PTX, a blocker of G protein activation, 100 ng/mL) , but not by glucocorticoid receptor antagonist RU38486 (10μM). In addition, cycloheximide (CHX, protein-synthesis inhibitor, 10μg/mL) pretreatment could not impair the Dex -evoked [Ca2+]i elevation. Conclusion These observations suggest that a nongenomic pathways may be involved in the effect of Dex on [Ca2+]i in cultured dorsal spinal cord astrocytes and the effect is mediated by a putative G-protein-coupled me