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宝鸡文理学院化学化工学院毕业论文 题目:萘啶类荧光小分子在缺陷DNA腔体内荧光行为的研究 学生姓名 指导教师 班级 专 业 学 院 目 录 摘要 1 关键词 1 Abstract ……………………………………………………………………… ………………...…..1 Keywords…………………………….………………………………………………..……...….. 2 1 前言 3 2 实验部分 4 2.1 主要试剂及仪器 4 2.2 荧光光谱的测量 4 3 结果和讨论 5 3.1 非标记分子信标的设计 5 3.2 GSMB探针的表征 5 3.3 使用非标记分子信标检测目标DNA 8 4 结论 9 参考文献 11 谢 辞 12 萘啶类荧光小分子在缺陷DNA腔体内荧光行为的研究 杨世明( (宝鸡文理学院 化学化工学院，陕西 宝鸡 721007) 摘 要：本文主要研究荧光小分子作为信号物质用于构建信号增强型非标记分子信标。利用两段单链DNA杂交形成一个发卡DNA结构，发卡DNA的茎状部分形成了一个空缺位点，空缺位点对面是C碱基。荧光杂环小分ATMND通过氢键识别未配对的C碱基并嵌入空缺位点，ATMND荧光发生淬灭。当向体系加入与分子信标环状部分互补的目标连时，分子信标环状部分打开，ATMND与目标碱基结合的微环境发生变化，ATMND被释放出，其荧光强度恢复表明目标链的存在。研究了影响荧光淬灭的因素，优化了实验条件。研究发现，当加入目标DNA链时，ATMND荧光强度恢复，而加入含有错配碱基的目标链时，荧光恢复不明显，表明我们的非标记分子信标可以有效的区分完全互补链和单碱基错配。 关键词：2-氨基-5,6,7-三甲基-1,8-萘啶; 荧光; 空缺位点 Label-free fluorescent molecular beacon based on a small fluorescent molecule non-covalently bound to the intentional gap site in the stem moiety Yang Shi-ming ( College of Chem. Chem. Eng., Baoji University of Arts Sciences, Baoji 721007 Shaanxi ) Abstract：A label-free ?uorescent molecular beacon (MB) based on a ?uorescent molecule, 5,6,7-trimethyl-1,8-naphthyridin-2-ylamine (ATMND) which is non-covalently bound to the intentional gap site in the stem moiety of the label-free MB, was developed. a label-free molecular beacon (MB) based on non-covalent binding of a fluorescence molecule, 5,6,7-Trimethyl- 1,8-naphthyridin-2-ylamine (ATMND), to the intentional gap site in the stem moiety of a hairpin DNA has been developed. When the free-labeled MB was under a closed state, a gap site was formed in the stem moiety of a hairpin DNA. The fluorescence of the small fluorescence molecule was significantly quenched by binding to the unpaired base at the gap site through hydrogen bond, and thus the free-labeled MB shows almost no fluorescence. Upon hybridization with a complementary target ss-DNA (cDNA), the free-labeled MB undergoes a conformational change to take an open state, resulting in an effective fluorescence