专业英及语细胞培养.pptVIP

Cell Culture Maolie liujing Outline Introduction　 The Physical Environment of Cell Culture Standard Cell Culture Techniques Contamination(污染) in Cell Culture introduction Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. But in practice it refers to the culturing of cells derived from animal cells The History of Cell Culture In 1912, Alexis Carrel, attempted to improve the state of the art of animal cell culture with experiments on the culture of chick embryo tissue. Carrel succeeded in expanding the possibilities of cell culture by keeping fragments of chick embryo heart alive and beating into the third month of culture and growing chick embryo connective tissue for over 3 months. This, along with Harry Eagles (1955) demonstration that the complex tissue extracts, clots, and so forth previously used to grow cells, opened up a new area of cell culture. The demonstration that each cell type has an optimal mix of nutrients that supports its function (Ham and McKeehan, 1979; Waymouth, 1981) has led to media derived to support specific cell types under specialized conditions. Finally, the advent of recombinant expression in mammalian cells and the creation of antibody-producing hybridoma(杂交瘤) cell lines, coupled with the use of large-scale culture techniques for culturing mammalian cells. A photograph of an industrial large-scale culture facility used to make recombinant proteins in mammalian cell culture. (A) Primary culture of isolated granulosa cells(粒层细胞) ;(B) a coculture of several cell types from neonatal(新生的) rat lung;(C) a follicle(卵泡) derived from coculture of rat immature granulosa cells and oocytes(卵母细胞) that reassociate in vitro. The Physical Environment of Cell Culture Laboratory The essential idea of the entire panoply of the chemical solution and mechanical apparatus surrounding the cell in vitro