基于荧光图像的抗肿瘤细胞迁移药物筛选方法Development-药学学报.PDFVIP

药学学报 Acta Pharmaceutica Sinica 20 11, 46 (7) : 793797 ·793 · 基于荧光图像的抗肿瘤细胞迁移药物筛选方法 1 2 1* 聂晓静 , 赵筱萍 , 王 毅 ( 1. 浙江大学 学院, 浙江 杭州 310058; 2. 浙江中医 大学, 浙江 杭州 310053) 摘要: 本文建立了一种基于荧光图像的抗肿瘤细胞迁移 物筛选方法。应用Hoechst 33342 荧光染料染色和 96 孔Transwell 板, 通过细胞荧光图像采集与分析平台对正常组和加 组细胞荧光图像进行定量分析, 建立了一 种快速筛选抗肿瘤细胞迁移 物的新方法。对本方法的稳定性、精密度、荧光染色时间和迁移时间进行了考察 和优化, 并对抗肿瘤 紫杉醇的肿瘤细胞迁移抑制作用进行了定量分析, 计算得到紫杉醇抗肿瘤细胞迁移的 IC50 为0.717 mol·L−1 。采用该方法对中 泽泻24 个组分进行筛选, 发现1 个活性组分。结果表明, 该方法快速、 准确、重现性好, 能够用于抗肿瘤细胞迁移药物的快速筛选。 关键词: 肿瘤细胞迁移; 荧光成像; 高通量筛选; 泽泻 中图分类号: R965.1 文献标识码:A 文章编号: 0513-4870 (20 11) 07-0793-05 Development of fluorescence imaging based assay for screening compounds with anti-migration activity 1 2 1* NIE Xiao-ji ng , ZHAO Xiao-ping , WANG Yi (1. College of Pharmaceutical Sciences of Zhej iang University, Hangzhou 310058, China; 2. Zhej iang Chinese Medical University, Hangzhou 310053, China) Abstract: In the present study, A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates. The correlation, precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized. In addition, The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.7 17 mol·L−1 was obtained. Using this method,