枯草芽孢杆菌胞外氨肽酶对菌体生长的作用研究.PDFVIP

微生物学通报 Sep. 20, 2015, 42(9): 1698−1702 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.150228 研究报告 枯草芽孢杆菌胞外氨肽酶对菌体生长的作用研究 1 2 2 2* 房月芹 高新星 周哲敏 刘中美 (1. 江南大学 环境与土木工程学院 江苏 无锡 214122) (2. 江南大学 生物工程学院 江苏 无锡 214122) 摘 要：【目的】利用基因敲除技术构建突变菌株BS-AP-K 来研究枯草芽孢杆菌的分泌型氨肽酶 对菌体生长的作用。【方法】基于Xer/dif 重组系统敲除Bacillus subtilis 168 基因组中ywaD 基因， 研究比较野生型与BS-AP-K 菌株在不同培养基中的生长情况。【结果】通过比较两菌株的生长情 况，发现敲除分泌型氨肽酶会对菌体生长带来不利影响，而这种影响可以通过在培养基中添加多 种游离氨基酸来弥补。【结论】研究结果表明胞外氨肽酶通过酶切外源蛋白质以及多肽来为细胞 生长提供营养所需。 关键词：枯草芽孢杆菌，氨肽酶，Xer/dif 重组系统，基因敲除 Effect of secretory aminopeptidase in Bacillus subtilis on the growth FANG Yue-Qin1 GAO Xin-Xing2 ZHOU Zhe-Min2 LIU Zhong-Mei2* (1. School of Environment and Civil Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China) (2. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China) Abstract: [Objective] Aim to study the effect of secretory aminopeptidase in Bacillus subtilis 168 on the growth, a gene knock-out mutant BS-AP-K was constructed. [Methods] Based on the Xer/dif recombinant system, the ywaD gene was deleted from the genome. The growth of wild type and BS-AP-K in different media was monitored. [Results] It turned out that the deletion of secretory aminopeptidase had a negative effect on the growth of the strain. The impaired growth rate of BS-AP-K strain can be overcome by amino acid supplements. [Conclusion] This study indicated that the secretory a