蛋白质结构与功能的NMR研究-2.pptVIP

4. Ssh10b与核酸相互作用温度依赖性的结构基础 Ssh10b在核磁谱图上呈现两种象，比例 与温度相关．谱图分析表明两种构象是 L61-P62顺反异构造成的 L61-P62 反式 顺式 在新药发现中的NMR 应用 An important technique in support of structure-based drug design provide information on the nature of molecular interactions, identify weak-binding compounds to aid development of the drug-like inhibitors for use as lead compounds in drug discovery. NMR进行新药研究的策略 检测蛋白质： 1. 选择性地同位素标记某种类型的氨基酸残基; 2. ‘segmental labelling’: discrete segments of the polypeptide chain are uniformly labelled; 3. Chemical-shift mapping or Differential chemical-shift mapping 检测底物小分子： Based on T2 and T1