云南切梢小蠹气味结合蛋白的同源模建-应用昆虫学报.PDFVIP

Chinese Journal of Applied Entomology 2015, 52(5): 12231228. DOI: 10.7679/j.issn.20951353.2015.145 云南切梢小蠹气味结合蛋白的同源模建* 1** 2 1 1 1*** 汪 宇 孙 浩 朱家颖 赵 宁 杨 斌 1. 650224 2. 650224 摘 要 【目的】Tomicus yunnanensis Odorant binding proteins OBPs OBP 【方法】OBP ProcheckVerify_3D ERRAT 【结果】6 ProcheckVerify_3D ERRAT 【结论】 关键词 Homology modeling of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis 1** 2 1 1 1*** WANG Yu SUN Hao ZHU Jia-Ying ZHAO Ning YANG Bin (1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China; 2. College of Science, Southwest Forestry University, Kunming 650224, China) Abstract [Objectives] This study aims to provide the basis for revealing the mechanism of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis involved in olfactory reorganization [Methods] The three dimensional structure of T. yunnanensis’s OBP was constructed using the homology modeling method and the reliability of the model was evaluated by Procheck, Verify_3D and ERRATERRAT. [Results] Analysis of the optimized structure found that the OBP of T. yunnanensis contains 6 conservative cysteine residues in its sequence. Evaluation of the model by Procheck, Verify_3D and ERRATERRAT indicated that it is reasonable and reliable. [Conclusion] The results showed that it is possible to predict the structure of a protein that has a known sequence with unknown spatial structure based on known structural information of its homologous proteins. The results