第01单元：力达霉素经线粒体依赖通路介导细胞凋亡.pdfVIP

132 Acta Pharm aceutica Sin ica 2007, 42( 2): 132- 138 2 1 1 1* 邱 强 , 王 真 , 蒋建明, 李电东 ( 1. , 100050; 2. , 100853) : ( lidamycin, LDM ), , BEL-7402 MCF-7 M ; c Bax, Bcl-2 p53; R -PCRp53 bax mRNA, LDM , 2 h c Bc-l 2 Bax , Bcl-2p53 6 hBaxmRNA , p53Caspase LDM LDM c : ; ; ; : R963; R978. 1 : A : 0513- 4870( 2007) 02- 0132- 07 Effect of lidam ycin on m itochondria initiated apoptotic pathway in human cancer cells 2 1 1 1* Q IU Q iang, WANG Zhen , JIANG Jian-m ing, LID ian-dong (1. Institute of M edicinalB iotechnology, ChineseA cademy of M edical Sciences and eking UnionM ed ical College, B eijing 100050, Ch ina; 2. D ep artment of N ep hrology, LA H osp ital, Beijing 100853, China) Abstract: A lthough enediyne antibiotic lidamycin ( LDM ) is a potent inducer of apoptosis, the underlying m echanism s of its apoptotic functions remain to be explored. H ere, we ami to elucidate its possible mechanisms in m itochondria initiated apoptotic pathway involved in hum an BEL-7402 andMCF-7 cells. Cytochrome c released from m itchondria to cytosol fraction was detected byW estern blotting. p53 and Bax, Bc-l 2 expressionswere detected byW estern blotting andR -PCR. M assay wasused to detect cytotoxicity of LDM w ith orw ithout caspase inhibitor z-VAD-fmk. After the BEL-7402 cellswere exposed - 1 to 01 mol L LDM w ithin 6 h, the increase of cytochrom e c in the cytosol and decrease in the m itochondria were observed when compared w ith untreated cells. he expression of Bax, an mi portant proapoptoticmember of the Bcl-2 fam ily, increased gradua