棉花4个GL2同源基因的序列比较及表达分析-作物学报.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2008, 34(6): 1086−1091 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.01086 棉花4 个GL2 同源基因的序列比较及表达分析 杨 霞 侯 磊 肖月华 李德谋 张国芳 裴 炎* ( / , 400715) : GLABRA2(AtGL2) EST 4 AtGL2 (GhHOX1GhHOX2GhHOX3 GhHOX4) 4 HOX AtGL2 Homeobox 95% 71% 72%63%RT-PCR , GhHOX1 GhHOX3 GhHOX4 ; GhHOX3 GL2 : ; GL2 ; Real-Time RT-PCR Sequence Analysis and Characterization of Four GL2 Homologous Genes in Cotton (Gossypium hirsutum L.) * YANG Xia, HOU Lei, XIAO Yue-Hua, LI De-Mou, ZHANG Guo-Fang, and PEI Yan (Biotechnology Research Center / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Southwest University, Chongqing 400715, China) Abstract: Arabidopsis GLABRA2 (AtGL2), a transcription factor, plays critical roles in the differentiation of trichome cells and root hair cells. However, the function of GL2 homologous genes in cotton fiber differentiation and development is still unclear. In this paper, transcription profiles of four putative cotton HOX ORFs homologous with AtGL2 were investigated. Multiple sequence alignment revealed that these 4 GhHOX proteins shared 95%, 71%, 72%, and 63% identity with AtGL2 in the homeobox con- served domain, respectively. Real-time RT-PCR analysis indicated that GhHOX1, GhHOX3 and GhHOX4 expressed in cotton elongating fiber cells preferentially. Moreover, GhHOX3 showed significant higher expression level in the ovules of Xuzhou 142 than in its fuzz-free and lint-free mutant ovules at the initiation stage. The results suggested that the GL2 homologous genes of cotton may play important roles in fiber initiation and elongation. Keywords: Cotton; Gl2 homologous genes; Real-time RT-PCR (Gossypium hirsutum L.)