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细胞生物学试验指导-湖北师范大学生命科学学院
细胞生物学实验指导
王卫东 江涓 编著
2008 年 3 月
湖北师范学院生物学实验教学中心 细胞生物学实验指导
目 录
前 言 ··························································································································· 2
细胞生物学实验守则 ···································································································· 3
实验一：动物细胞的基本形态观察和细胞计数························································· 5
实验二：细胞凝集反应和细胞膜通透性的观察························································· 7
实验三：聚乙二醇（PEG ）介导的细胞融合····························································· 9
实验四：线粒体和液泡系的超活染色与观察··························································· 11
实验五：植物细胞微丝束的光学显微镜观察··························································· 12
实验六：叶绿体的分离与荧光观察 ·········································································· 14
实验七：DNA 的显示——Feulgen反应 ····································································· 17
实验八：小鼠腹腔巨噬细胞吞噬现象的观察··························································· 20
实验九：小鼠骨髓染色体标本的制备与观察··························································· 22
实验十：染色体C-带技术 ·························································································· 25
实验十一：细胞内化学成分的显示方法——酸性磷酸酶的显示 ··························· 26
实验十二：开放性实验——蚕豆 （Vicia faba）根尖细胞微核监测技术··············· 28
细胞生物学实验考试大纲 ·························································································· 32
实验考试试题示例·································································
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