奶山羊乳腺发育过程中腺苷酸质量分数及能荷的变化.pdfVIP

wvcvr．chinadairy．net 中国乳品工业 rPg)r@chinajournal．net．cll 奶山羊乳腺发育过程中腺苷酸质量分数及能荷的变化 张娜，李庆章 (东北农业大学乳品科学教育部重点实验室，哈尔滨150030) 摘要：探讨了奶山羊乳腺发育和泌乳过程中能量代谢变化规律和特点。分别在奶山羊乳腺发育的4个时期青春期、妊娠期、泌乳期及 退化期采集乳腺组织。应用高效液相色谱测定各时期的腺苷酸质量分数并计算出各时期能荷：用总ATP酶活性测定试剂盒测定各时期 AMP比例急剧减少，能荷值恢复到正常水平。在乳腺发育过程中，泌乳启动后乳腺中ATP酶活性急剧增加，腺苷酸质量分数增加；泌乳 高峰期过后ATP酶活性下降，腺苷酸质量分数减少。 关键词：奶山羊；乳腺；能荷；腺苷酸库 中图分类号：TS827．9+4文献标识码：A 文章编号：1001—2230{2009)09-0014—03 ofthe and inthe ofmilk Changesadenylatepool energycharge development goatmammarygland ZHANG Na，LI Qing—zhang of Scienceof of (TheKeyLaboratoryDairy MinistryEducation，NortheastAgriculturalUniversity， Harbin150030，China) Abstract：Tothe metabolisminthe ofmilk of acid and investigateenergy goatmammaryglalldbystudyadenylicpool energy development in ofChinese milk based012the characterWaSdividedinto4 chargemammarygland．Themammarygland Guanzhonggoats development pe- and sizeof acid was andmea- involution．The mammary separated riods，virgin，pregnant，lactation adenylicpool(ATP，ADP，AMP)ingland sured ATPase of Wasmeasured and high activitymammary舀and byKits．adenylatepool by performanceliquidchromatography(HPLC)．The ATPase increasedin thedifferencewasnot as activity slowlyvi咖andpregnantperiod，but significant．ATPaseactivitysharp ADPandAMP Lactation．Atthe of andAMPcontent contentWasincreasedafterthecommencementof hctaf