小反扭藓原丝体的发育特征与油体形态研究.pdfVIP

中国农学通报 2010,26(11): 144-147 Chinese Agricultural Science Bulletin 小反扭藓原丝体的发育特征与油体形态研究 于淑玲 （邢台学院生物化学系，河北邢台054001） 摘 要：探讨小反扭藓原丝体的发育规律，为研究该藓的个体发育、人工培养和探讨生活史提供新的数据 和资料。在配置改良的Knop 营养液，接种用75%的酒精溶液消毒3 min 孢蒴的孢子，选取孢子萌发、原 丝体发育和配子体发生过程中的9 个主要特征，对小反扭藓的原丝体发育特征进行了分析，并进行显微 照相。结果表明，孢子第2 天开始萌发，6 天时，孢子的萌发率已经达到90% 。原丝体系统由绿丝体、轴 丝体和泡状原丝体组成。原丝体发育过程中形成空细胞使原丝体断裂，无假根；油体的大小没有大的变 化，位置发生了移动。此藓孢子的萌发类型为光藓型，原丝体的发育特征是其对生长环境长期适应的结 果。 关键词：原丝体；绿丝体；轴丝体；假根；发育 中图分类号：Q944 文献标志码：A 论文编号：2009-2732 Experimental Studies on the Characteristics of Protonema and Observation of the Oil Body Development in Timmiella diminuta (C. Mull.) chen YuShuling (Biology Department, Xingtai College, XingtaiHebei054001) Abstract: This paper provides new data and information to study the law of the development of small anti-twistedontogenymossandexploreitslifecycleandartificialcultivation.PutinthemodifiedKnops nutrientsolutionwith75%alcoholsolutionof3minsporecapsuleofthespores,sporegerminationwasselected intheprocessoftheninemainfeatures,whichwereanalyzedandmicrographed.Theresultsshowedthat sporesbegantogrowinthefirsttwodays.Atthesixthday,thesporegerminationratehadreached 90%. Protonemasystemwascomposedofgreensilkbody,axialfilamentbodyandbubble-likeprotonrma.Empty cellswereformedwithfracture,andwithoutrhizoid.Inthedevelopmentprocesslocationwasshifted,nomain changesoccurredaboutthesizeofoilbodies.Thistypeofsporegerminationwasclassifiedasmosstype.The charactersofthegrowthofprotonemaweretheresultsoflong-termadaptation. Key words:protonema;chloronema;caulonema;rhizoid;development [1] 0 引言 物