2016七年度制-凋亡 衰老.pptVIP

4. Mechanisms ICE gene family (caspase family） bcl-2 gene family P53 Fas, Fasl Ced gene family C. elegans 秀丽线虫 1090 131 ced3ced4 normal cell Determinates to die perform death a cell that is informed to die ced9 cell death supressor gene cell death gene dead cell phagocytosis degradation a cell being phagocytosed Caspase family—ced-3 homogene Bcl-2 gene family---ced-9 homogene Methods Staining by HE, AO(丫啶橙), Typan blue, Giemsa etc. EM Electrophoresis FCM TUNEL Key points Cell senescence Hayflick limitation Apoptosis Morphological and biological events of apoptosis(DNA ladder) Gene