附红细胞体感染小鼠Thl7 细胞的表达.pdfVIP

中国农学通报 2012,28(05):62-66 Chinese Agricultural Science Bulletin 附红细胞体感染小鼠Th17 细胞的表达 杨蕾芳，巴彩凤 (辽宁医学院实验动物中心，辽宁锦州121001) 摘 要：为分析附红细胞体感染后Th 17 细胞所发挥的免疫功能。采用RT-PCR 及荧光抗体双标的方法检 测小鼠脾脏IL- 17 的表达变化。结果显示：小鼠感染附红细胞体后，IL- 17表达水平显著增高，提示Th 17 细胞在附红细胞体感染后发挥了重要的免疫作用，为该病的临床诊断、治疗及预防提供了理论依据。 关键词：附红细胞体；RT-PCR ；荧光抗体双标；Th 17 细胞 中图分类号：R3 文献标志码：A 论文编号：2011-3119 The Expression of Th17 Cells for the Mice with Eperythrozoon Yang Leifang, Ba Caifeng (Experimental Animal Center, Liaoning Medical College, Jinzhou Liaoning 121001) Abstract: To investigate the immunologic function of Th 17 cells for the mice infected with Eperythrozoon (Mycoplasma suis), the RT-PCR assay and the fluorescence antibody technique were used to detect the variation of interleukin- 17(IL-17) in mousey spleen tissue. The results showed that the IL-17 expression level increased obviously when the mice infected with Eperythrozoon. It prompted that the Th17 cells participated in the immunoregulation, and played an important role in inflammation which the mice infected by Mycoplasma suis. Accordingly, this experimental research had offered some theoretical bases for the diagnosis, therapy and prevention about Eperythrozoonosis. Key words: Eperythrozoon; RT-PCR; fluorescence antibody technique; Th17 cells [3] 0 引言 具有重要的意义 。目前有关附红细胞体感染机体后 附红细胞体病(Ep erythrozoonosis) 是由寄生于 Th 17细胞发挥免疫调节的研究国内外报道尚属罕见， 人、动物红细胞表面、血浆及骨髓内的原核生物——附 本实验通过建立小鼠感染附红细胞体模型，观察小鼠 红细胞体(Ep erythrozoon) 引起的人兽共患传染性疾 T 细胞分化，检测Th 17 细胞表达水平变化，探讨Th 17 病，可以通过血液、消化道、垂