蜡状芽胞杆菌群代谢途径的分析和比较.pdfVIP

HEREDITAS (Beijing) 2011 10 , 33(10): 1057―1066 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2011.01057 蜡状芽胞杆菌群代谢途径的分析和比较 1 1 1 1 2 1 喻婵 , 王琰 , 徐承晨 , 何进 , 张青叶 , 喻子牛 1. , , 430070; 2. , 430070 微生物基因组测序和高通量分析方法获得了大量的数据和信息, 利用这些信息研究代谢网络成为当前 的一个新热点。文章在比较和分析重构代谢网络不同方法的基础上, 利用蜡状芽胞杆菌群中已测序的9 株蜡状 芽胞杆菌、6 株炭疽芽胞杆菌、6 株苏云金芽胞杆菌基因组, 对它们的碳水化合物代谢途径、氨基酸代谢途径 和能量代谢途径进行比较与分析, 找出它们的共性和特性。这3 种菌都存在必需的糖酵解、三羧酸循环、丙氨 酸代谢、组氨酸代谢及能量代谢等途径; 同时它们还存在特殊的代谢途径, 蜡状芽胞杆菌对单糖的利用率较高; 炭疽芽胞杆菌的氨基酸降解和转运途径较丰富; 苏云金芽胞杆菌中存在催化谷氨酸转化的代谢旁路等。代谢途 径的分析为深入研究它们的食物毒素、炭疽毒素和杀虫毒素提供了新思路。 蜡状芽胞杆菌群; 代谢网络; 代谢途径分析 Analyze and compare metabolic pathways of Bacillus cereus group 1 1 1 1 2 1 YU Chan , WANG Yan , XU Cheng-Chen , HE Jin , ZHANG Qing-Ye , YU Zi-Niu 1. State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbial Pesticides, Huazhong Agri- cultural University, Wuhan 430070, China; 2. School of Science, Huazhong Agricultural University, Wuhan 430070, China Abstract: A large number of data and information was obtained from genome sequencing and high-throughput genomic studies, use of the information to study metabolic networks become a new hotspot in biological research. This article com- pared different methods to reconstruct metabolic networks and analyzed the advantages and disadvantages of each methods, and then introduced some researches about carbohydrate metabolism pathways, amino acid metabolic pathways, and energy metabolism pathways of 9 strain