油菜蛋白质组学研究进展.pdfVIP

中国农学通报 2013,29(27):7-12 Chinese Agricultural Science Bulletin 油菜蛋白质组学研究进展 解 伟，陈社员，张振乾 （湖南农业大学农学院，长沙410128 ） 摘 要：蛋白质是生理功能的执行者，是生命现象的直接体现者，要对生命的复杂活动有全面和深入的认 识，就必须进行蛋白质组学方面的研究。菜籽油是中国重要的食用油，随着油菜产业的发展，出现了各 种不同的育种要求，为了达到这些目标，需要了解其分子机理，为此要加强油菜蛋白质组学方面的研 究。本研究总结了油菜蛋白质提取方法及双向电泳条件优化，营养元素、低温、盐和水等胁迫方面的研 究，种子、汁液、花粉、叶片、种皮、线粒体等不同组织材料的生理生化研究和抗病研究等方面的蛋白质组 学研究。指出目前的研究存在一些不足之处，如没有紧密联系生产需求、研究不系统、研究方法相对滞 后，如能够在今后的研究中加强，将会促进中国油菜产业的发展，保障中国的食用油安全。 关键词：油菜；蛋白质组学；质谱；双向电泳 中图分类号：S565.403.2 文献标志码：A 论文编号：2013-0372 The Advances on the Proteomics of Rape Xie Wei, Chen Sheyuan, Zhang Zhenqian (CollegeofAgriculture,HunanAgriculturalUniversity, Changsha 410128) Abstract: Protein is the executor of the physiological function, and it is the direct embodiment of the life phenomena. So, the proteomics research must be carried on to obtain a comprehensive and in-depth understanding of complex activities of life. Rapeseed oil is a very important to China, with the development of rape industry, a variety of breeding objectives are needed. Then, the study of rape proteomics must be strengthened for the further understanding of the molecular mechanism. This paper reviewed the study of the rape protein extraction methods and the operation conditions of two-dimensional electrophoresis, the study of stress on rape, such as nutrient, temperature, salt, water and so on, the study of physiological and biochemical on different rape materials, such as seed, pollen, juice, leaf, seed coat, mitochondria and so on, and the study of disease resistance of rape proteomics. Then some shortcomings were founded, such as the current study did not