胰岛素样生长因子受体Ⅰ序列结构的生物信息学分析.PDFVIP

生 物 工 程 学 报 麦秀英 等/胰岛素样生长因子受体 Ⅰ序列结构的生物信息学分析 693 Chinese Journal of Biotechnology /cjbcn May 25, 2016, 32(5): 693−701 DOI: 10.13345/j.cjb.150377 ©2016 Chin J Biotech, All rights reserved 生物技术与方法 麦秀英，李萍，徐柳 610031 , , . . , 2016, 32(5): 693–701. Mai XY, Li P, Xu L. Bioinformatics analysis of sequence and structure of insulin-like growth factor-I receptor. Chin J Biotech, 2016, 32(5): 693–701. 摘 要: 胰岛素样生长因子受体 Ⅰ的 3UTR 长度大于7 kb ，结构复杂，有多种miRNAs 的结合位点，参与信 号通路中MAPK 及 PI3K/AKT 的调节和多种肿瘤的形成和发展，通过生物信息学分析知道其结构特点，为后 续研究提供思路。分析表明儿童神经胶质瘤中IGF1R 的3UTR 与miRNAs 结合位点突变率最高。分析IGF1R 序列 3UTR 的结构，miRNAs 结合位点，氨基酸序列的理化性质，亲疏水性，糖基化和磷酸化位点，二级结构 和三级结构建模。IGF1R 三级结构与配体IGF1 的三级结构模拟分子对接，得到2 种蛋白相互作用的氨基酸位 置及名称。因此，通过对IGF1R 3UTR 突变，降低与miRNAs 的结合，IGF1R 表达上调，同时改变与IGF1 的 氨基酸结合位点，降低2 种蛋白的相互作用，从而抑制IGF1R 的作用。 : 胰岛素样生长因子受体I ，序列分析，三维建模，分子对接 Bioinformatics analysis of sequence and structure of insulin-like growth factor-I receptor Xiuying Mai, Ping Li, and Liu Xu School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, China Abstract: The length of IGF1R 3’UTR is greater than 7 kb. The structure of IGF1R 3’UTR is complex, with multiple binding sites of miRNAs. IGF1R is involved in the regulation of MAPK and PI3K/AKT signaling pathways and the formation and development of tumors. Bioinformatics analysis can reveal the structure features of IGF1R, which provides ideas for further research. The analysis shows that the binding sites between IGF1R and miRNAs have the highest mutation Received: August 25, 2015; Accepted: October 19, 2015 Supported by: Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Educati