人参茎叶皂甙对鸭禽流感疫苗的免疫增强作用和抗氧化功能的影响.docVIP

人参茎叶皂甙对鸭禽流感疫苗的免疫增强作用和抗氧化功能的影响 　　摘要：为探讨人参茎叶皂甙（GSLS）对鸭流感疫苗的免疫增强作用和抗氧化功能的影响，给鸭群饮水口服GSLS，同时接种禽流感疫苗，通过检测血清抗AIV特异性HI效价、免疫器官指数、淋巴细胞增殖转化指数、细胞因子和肠黏膜SIgA水平，评价GSLS的免疫增强作用；通过测定血清丙二醛（MDA）和超氧化物歧化酶（SOD）含量，评价GSLS对机体抗氧化功能的影响。结果表明：在28和35日龄时，饮水口服中剂量GSLS组鸭血清抗AIV特异性HI效价显著高于对照组（P0.05），1份+GSLS组鸭血清抗AIV特异性HI效价显著提高（P0.05）；饮水口服GSLS能显著增强鸭脾脏和法氏囊指数（P0.05），显著提高受ConA刺激产生的脾淋巴细胞增殖转化指数，显著或不显著提高脾淋巴细胞上清中IFN-γ（Th1）和IL-4（Th2）水平，显著提高鸭肠黏膜SIgA含量；饮水口服中剂量GSLS组血清MDA和SOD含量分别显著低于和高于对照组（P0.05）。因此，饮水口服GSLS具有增强鸭群对禽流感疫苗的免疫应答水平和机体抗氧化功能的作用。 　　关键词：禽流感疫苗；人参茎叶皂甙；肉鸭；免疫增强作用；抗氧化功能 　　AbstractTo discuss the effects of GSLS on immunopotentiation to AI vaccine and antioxidation of body， the ducks were orally-administrated GSLS and then subcutaneously immunized with AI vaccine. The blood samples were collected to detect the specific hemagglutination inhibition （HI） titers， immune organs index， lymphocyte proliferation and transformation index， cell factor and intestinal mucosa SIgA level， then the immunopotentiation of GSLS was evaluated. The contents of malondialdehyde （MDA） and superoxide dismutase（SOD） in serum were detected to evaluate the effects of GSLS on body antioxidation. The results showed that compared with the conventional breeding management， orally-administrated middle-dose GSLS group could significantly increase the serum HI titer of 28-and 35- day-old ducks， significantly decreased the content of MDA， and significantly increased the level of SOD in ducks injected with AI vaccine. Compared with the one dose of vaccine， orally-administrated GSLS could improve the HI titer of 0.75 dose of vaccine without significant difference and significantly improve the HI titer of one dose of vaccine after immunized for 2， 3 and 4 weeks. Compared with the group immunized with AI vaccine alone， orally-administrated GSLS could significantly improve the indexes of spleen and bursa of Fabricius， splenic lymphocyte proliferation and transformation index stimulated by ConA， secretion of SIgA on intestinal， and significantly or not