术前检测乳腺癌雌孕激素受体方法的比较.docVIP

术前检测乳腺癌雌孕激素受体方法的比较 刘喜波1 孙爱静1 田井奇2 王宁1 孙丽萍1（绍兴市人民医院绍兴文理学院附属第一医院 1病理科, 2乳腺科 浙江 绍兴 312000） 摘要：目的 探讨液基细胞制片技术在乳腺癌细针穿刺细胞学标本中检测ER和PR的应用价值，尝试提高免疫细胞化学染色质量。方法：对50例乳腺癌细针穿刺细胞学标本分别进行液基细胞制片和直接涂片检查，然后分别进行免疫细胞化学染色检测ER和PR的表达水平，用术后石蜡切片免疫组织化学检测结果作对照。比较ER和PR的阳性率是否存在差异并对制片效果进行评价。结果：液基细胞涂片、直接涂片法和石蜡切片的ER阳性表达率分别为54%（27/50）、50%（25/50）和56%（28/50），三种方法之间无显著性差异（χ2 ＝0.375，P0.05）。PR的阳性率分别为44%（22/50）、38%（19/50）和52%（26/50），三种方法之间无显著性差异（χ2 =1.996, P0.05）。液基细胞涂片中背景清晰，而直接涂片中背景普遍存在红细胞。结论：液基细胞涂片和直接涂片进行免疫细胞化学染色检测术前乳腺癌针吸细胞标本中ER和PR的阳性率与术后免疫组织化学结果均无明显差异，可作为乳腺癌术前治疗的重要参考指标；液基细胞学法免疫细胞化学制片效果优于直接涂片法。 关键词： 液基细胞；免疫细胞化学；乳腺肿瘤；雌激素受体；孕激素受体 中图分类号: R365 文献标识码 :A Comparison of differently preoperative detection methods for estrogen and progesterone receptor in breast cancer LIU Xi-bo1, SUN Ai-jing1, TIAN Jing-qi2, WANG Ning1 ,SUN Li-ping1 （1 Department of Pathology, 2Department of Breast Surgery, Shaoxing People’s Hospital the First Affiliated Hospital of Shaoxing University, Shaoxing 312000, China） Abstract： Objective To explore the application value of liquid-based cytology technique to estrogen receptor (ER) and progesterone receptor (PR) detection in fine-needle aspiration cytology specimens of breast cancer and to improve the quality of immunocytochemistry staining. Methods ER and PR were detected by immunocytochemistry staining after liquid-based cytology production and direct smear in 50 cases of breast cancers fine-needle aspiration cytology specimens, then compared with which by immunohistochemistry staining on paraffin section. Difference of ER and PR expressions was analyzed between the different methods and quality of production was evaluated. Results ER positive rates were 54%(27/50) on liquid-based cytology smear , 50%(25/50) on direct smear and 56%(28/50) on paraffin section, without statistically significant difference among them (χ2 ＝0.375，P0.05), and PR positive rates were 44%(22/50), 38%(19/50) and 52%(26/50) on liquid-based cytology smear , direct smear and paraffin section, respectively, with