基于云南臭蛙抗菌肽序列特性.docVIP

摘要目的分析抗菌肽结构与活性之间的关系，提出一种抗菌肽类药物设计策略，为以后抗菌肽分子的设计和改造提供理论基础。方法采用生物信息学的方法，分别从一级结构、二级结构、导肽、信号肽、功能域、保守结构域、疏水/亲水性分析和多序列比对等方面对9种云南臭蛙抗菌肽进行序列特性分析，并以这些抗菌肽序列为模板进行设计和改造。结果云南臭蛙成熟肽均为带正电荷的疏水性蛋白，α螺旋是其二级结构的主要元件该类肽的C端均具有保守的Rana-box环状结构。GL(A/V/I)AANFLPK(T)AF(L)C XXXK(R)KC。[结论抗菌肽具备抗菌活性的主要条件对成熟肽序列进行氨基酸残基替代或肽链截短，关键词云南臭蛙；抗菌肽；生物信息学；设计Drug design strategy based on sequence characteristic analysis of Rana andersonii antimicrobial peptides Abstract：Objective] To analyze the relationship between the structure and activity of antibacterial peptides, put forward a design strategy of antibacterial peptide drugs, providing a theoretical basis for the future design and modification of the peptide molecules. [Method] Nine kinds of Rana andersonii antibacterial peptides were analyzed by using the bioinformatics tools, including the structure of primary and secondary, target and signal peptide, functional domain, conserved domain, hydrophobic/hydrophilic and multiple sequence alignment. [Results]Mature peptides from Rana andersonii are positively charged hydrophobic protein and α-helix is the main component of their secondary structure. Such peptides all have conservative Rana-box ring structure on their C-terminal. Based on the results, a peptide: GL (A/V/I) AANFLPK (T)AF(L)CXXXK(R)KC may have strong activity was synthesized artificially. [Conlusion]α-helix structure and positive charge of the peptide are the main factors to the peptides’ activity. It is a shortcut to obtain the peptide like drugs by amino acid residue substitution or peptide chain truncation. Key words: Rana andersonii; Antibacterial peptide; Bioinformatics; Drug design strategy 目 录 绪论…………………………………………………………………………………………………………1 第1章 材料与方法…………………………………………………………………………………………1.1 材料………………………………………………………………………………………………1.2 方法………………………………………………………………………………………………第2章 结果与分析…………………………………………………………………………………………2.1 云南臭蛙抗菌肽前体的一级结构分析…………………………………………………………5 2.2 云南臭蛙抗菌肽前体的二级结构分析…………………………………………………………2.3 导肽的预测和分析………………………………………………………………………………2.4