海藻糖负载对人红细胞冷冻干燥保存影响的实验研究.pdfVIP

28 中国输血杂志2oo8年1月第21卷第1期 Chin J Blood Transfusiondanuary，2008．Vo1．21。No．1 论 著 · 海藻糖负载对人红细胞冷冻干燥 保存影响的实验研究术 陈麟凤 刘景汉 欧阳锡林 庄远 车辑 (解放军总医院输血科全军临床输血中心，北京100853) 摘要：目的 初步探讨红细胞冻干长期保存的有效方法，并比较冻干前海藻糖的负载与否对红细胞冻干保存效 果的影响。方法 实验设实验组(负载海藻糖冻干一复水后红细胞)：37(2，红细胞负载海藻糖7h后。采用主要成分 为含有15％聚乙烯吡咯烷酮(PvP)和150mmol／L海藻糖的缓冲液作为保护液，在设定的降温程序下冻干保存红细 胞；对照组：未负载海藻糖冻干一复水后红细胞。冻干结束后用37~(2的再水化液快速水化，检测2组的各项理化指 标。结果 实验组红细胞冻干再水化后RBC和Hb回收率要高于对照组(P0．05)；ATP酶和葡萄糖一6一磷酸脱氢 镁(G-6-PD)活性水平显著差异有统计学意义(P0．05))。结论 胞内海藻糖对红细胞冻干具有明显的保护作用． 红细胞在37(2孵育7h的条件负载海藻糖后冻干一复水后能保持细胞的理化稳定性。 关键词：海藻糖；负载缓冲液；红细胞：冻干保存：PvP：复水 中图分类号：R．457．1 2 文献标识码：A 文章编号：1004—549X(2008)01—0028—04 Effects oftrehalose loading on the freeze-dried red blood cells CHEN Linfeng,LIUJinghan。OUYANG Xilin。et a1．De— partment ofBlood Transfusion，Center for Mihtary Clinical Blood Transfusion，Chinese PLA GeneraI Hospital。BeiJing 100853，China Abstract：Objective To find an efficient way for long—term preservation of red blood cells，and to evaluate the recov— ery and biological activities of the trehalose loaded and non—trehalose loaded RBCs after freeze—drying．Methods The sam— ples were divided into two groups：the control group(without loading RBCs in trehalose buffer)，and the test group(1oading RBCs in trehalose buffer)．We loaded RBCs at 37oC for 7 hours，then put the RBCs into the protective solution。which was mainly composed of 1 50mmol／L trehalose，1 5％polyvinylpyrrolidone(PVP)and isotonic buffer，and then lyophilized red blood cells by designed procedures．ARer freeze—drying．the samples were quickly rehydrated by 37℃ rehydration SO— lution．RBCs recovery and the activity levels of ATP and G一6一PD were investigated．Kesults The recovery of RBCs with or without intracellular trehalose aRer freeze—drying and rehydration were over 70％．and there was no significant difference in MHC，MCH