精品9 多酚氧化酶,过氧化物酶,脂肪氧合酶.ppt

On-line simplified methods for enzyme activity check Peroxidase test a) Solutions. In order to check the peroxidase activity two solutions have to be prepared: 1% guaiacol in alcohol solution (1 g guaiacol is dissolved in about 50 cm3 of 96% ethylic alcohol and then this preparation is brought to 100 cm° with the same solvent); peroxide solution 0.3% (1 cm3 perhydrol is brought to 100 cm3 with distilled water. b) Sampling. From various parts of the material samples are taken (about 20-30 pieces, etc.); the material is then crushed in a laboratory bowl in order to obtain an average sample. c