河北西瓜枯萎病菌生理小种鉴定与AFLP分析.DOC

河北省西瓜枯萎病菌生理小种鉴定与AFLP分析 段会军张彩英李喜焕郭小敏王省芬马峙英（河北农业大学/河北省作物种质资源重点实验室，保定 071001） Identification of Physiological Races and AFLP Analysis of Fusarium oxyporum f. sp. niveum from Hebei Province DUAN Hui-jun, ZHANG Cai-ying, LI Xi-huan, GUO Xiao-min, WANG Xing-fen, MA Zhi-ying (Agricultural University of Hebei，Key aboratory of Crop Germplasm Resources of Hebei, Baoding 071001) Abstract: 【Objective】To clear the types of physiological races and genetics diversity of Fusarium oxysporum f. sp. Niveum in Hebei Province, and provide the basis for disease-resistant breeding and integrated control of watermelon wilt.【Method】 isolates of Fusarium oxysporum f. sp. niveum from 12 watermelon growing areas of Hebei Province and 4 identified physiological races were tested to identify their pathogenicity and analyze genetic diversity by using AFLP technology.【Result】 Isolates46 of them) were classified into three physiological races according to the reaction types on three identified hosts, namely race 0 (17.4 %)、1 (65.2 %) and race 2 (17.4 %), respectively. Race 1 was predominant, and distributed all the watermelon growing areas. Using 21 pairs of AFLP primer pairs, a total of 1 157 bands were amplified, of which, 389 (33.6%) were polymorphic. Genetic distance of 46 isolates based on AFLP markers ranged from 0.33 to 0.95, averaged 0.66. This showed that the genetic diversity was high among the tested isolates. When all of polymorphic bands were analyzed by clustering, 50 isolates were classified into 3 groups (AFLP groups, abbreviated AGs). AGI and AGII included 4 race2 and 4 race 0, respectively. In AGIII, among the 42 isolates, 32(accounted for 76.2%) belonged to physiological race 1. 【Conclusion】The results showed that there existed obvious differentiation of the pathogenicity of Fusarium oxysporum f. sp. niveum in Hebei Province, and AFLP groups were generally related with the different races. However, there was no relationship between the AFLP groups and geographic regions of isol