中国热带作物研究院香蕉.pdfVIP

Planta DOI 10.1007/s00425-007-0502-6 ORIGINAL ARTICLE DiVerentially expressed cDNAs at the early stage of banana ripening identiWed by suppression subtractive hybridization and cDNA microarray Bi Yu Xu · Wei Su · Ju Hua Liu · Jia Bao Wang · Zhi Qiang Jin Received: 6 February 2007 / Accepted: 14 February 2007 © Springer-Verlag 2007 Abstract The banana (Musa acuminate L. AAA signal transduction; transport and intracellular traYc; group) fruit undergoes a postharvest ripening process, cell structure, growth, and division; transcription and which plays an important role in improving the quality post-transcription; and disease and defense. To charac- and extending the shelf life of bananas. To manipulate terize diVerentially expressed cDNAs in the SSH library, postharvest banana ripening, a better understanding of cDNA microarray analysis was conducted. A total of the mechanism of postharvest ripening is necessary. 26 cDNAs in the 2-DPH banana fruit were found to be The isolation of mRNA transcripts encoding proteins up-regulated and these results were conWrmed by associated with the ripening process is a powerful tool using reverse transcriptase-polymerase chain reaction for this purpose. To isolate diVerentially expressed (RT-PCR). The information generated in this study pro- genes at the early stage of postharvest banana ripening, vides new clues to aid in the understanding of banana a forward suppression subtractive hybridization (SSH) ripening. cDNA library was constructed. SSH was performed with cDNA from banana fruit on the day of harvest as Keywords Banana (Musa AAA group cv Brazilian) · the “driver” and cDNA from banana fruit 2 days post- cDNA microarray · Postha