三七总皂苷促进脑出血后侧脑室室管膜下神经干细胞的与增殖和分化__.docVIP

三七总皂苷促进脑出血后侧脑室室管膜下神经干细胞的增殖和分化**☆ 司银楚，李巾伟，张丽娟，吴海霞，许 红，朱培纯 Panax notoginseng saponins promote the proliferation and differentiation of neural stem cells in subependymal zone of the lateral ventricle in rat brain after intracerebral hemorrhage Si Yin-chu, Li Jin-wei, Zhang Li-juan, Wu Hai-xia, Xu Hong, Zhu Pei-chun Abstract AIM: Panax notoginseng saponins can promote the expression of Nestin, proliferating cell nuclear antigen and basic fibroblast growth factor in subependymal zone of lateral ventricle in rat brain after cerebral hemorrhage, and accelerate the proliferation and differentiation of cortex neural stem cells of fetal rats. This article investigates the influence of panax notoginseng saponins on proliferation and differentiation of subependymal cells of lateral ventricle in rat brain after intracerebral hemorrhage by the collagenase method and immunohistochemical staining. METHODS: Experiments were performed at the Laboratory of Neuro-anatomy of Beijing University of Chinese Medicine from 2002 to 2006. ①Sixty clean male Wistar rats weighting 220-250 g were provided by Animal Experimental Center of China Academy of Chinese Medical Sciences. Rats were randomly assigned into a normal group, a model group and an administration group, 20 in each group. Animal intervention met the animal ethical standard. Panax notoginseny saponins Xueshuantong injection was offered by Inner Mongolia Kangyuan Yaoye Company, No. 22-5802 (1999) 1787, specification 5 mL: 175 mg. ②Rat models of cerebral hemorrhage were established with collagenase as a inductor. Rats in the administration group received 35 mg of panax notoginseny saponins intraperitoneally after the surgery, once a day. Rats in the model group received saline intraperitoneally after the surgery, once a day. Rats in the normal group did not receive any intervention. ③Proliferating cell nuclear antigen, β-Ⅲ tubulin and glial fibrillary acidic protein expression in ependyma was determined by the immunohistochemical