髓鞘少突胶质细胞糖蛋白MOG抗原肽与免疫原性验证.docVIP

ＸＸ大学毕业论文 题 目：髓鞘少突胶质细胞糖蛋白MOG抗原肽免疫原性验证 学生姓名： XXX 学号： XXXXXXXXX 指导教师： XXX 职称： 教授 专 业： 生物技术 院（系）： 生物工程系 完成时间： 2011年5月25日 2011年5月25日 摘要 目的： 通过实验性自身免疫性脑脊髓炎（Experimental autoimmune encephalomyelitis, EAE） Abstract Objective The model of experimental autoimmune encephalomyelitis(EAE) was established in female C57BL/6 mice to validate the immunogenicity of myelin oligodendrocyte glycoprotein (MOG), to observe pathological changes of EAE, and to explore the possible pathogenesis of multiple sclerosis(MS). Methods Each mouse was injected s.c over flanks with MOG35-55, Mycobacterium H37Ra, and CFA, injected i.p with pertussis toxin. The clinical symptoms were observed and the pathological changes of EAE were studied with the electron microscopy. Consecutive sections were stained with hematoxylin/eosin(HE) and Luxol Fast Blue(LFB), to assess inflammation and demyelination. Results The incidence of EAE in immunization animal reached 100%, most mice developed clinical EAE with in about 15 days of immunization MOG35~55 peptides. With Electron microscopy, inflammatory cells in white matter and demyelination were observed. And the component of axon is disappeared. Conclusion The immunogenicity of MOG35~55 synthesized by the improved solid-phase synthesis method was identified by the induction of EAE in C57BL/6 mice with stable performance and high incidence, which may be helpful in exploring the mechanism and therapy of Multiple sclerosis(MS). Key Words: Myelin oligodendrocyte glycoprotein(MOG), Experimental autoimmune encephalomyelitis(EAE), Mouse models, Pathological changes 目录 引言 1 一、材料和方法 2 1. 试剂和仪器 2 1.1. 主要试剂 2 1.2 主要仪器 2 2. 实验动物 2 2.1 小鼠来源 2 2.2 饲养 3 3. EAE小鼠模型的制备 3 4. 神经功能评分 3 5. 脊髓组织病理学检测取材 3 6. 溶液的配制 3 7. HE、LFB染色 4 7.1 HE染色 4 7.2 LFB染色 4 8. 数据分析及统计学处理 5 二、实验结果与分析 5 1. 临床评分 5 2 病理学检测结果 6 2.1 HE结果 6 2.2 LFB的结果 7 三、讨论 8 参考文献 10 致谢 12 引言 实验性自身免疫性脑脊髓炎（experimental autoimmune encep