1蛋白质高级结构.pdfVIP

2.3蛋白质的高级结构 1, Tools to investigate the protein conformation 2, Super secondary structures and domains 3, globular proteins and SCOP 4, fibrous proteins Helpful websites: 1, PDB (Protein Data Bank) /pdb/index.html 2, SCOP (Structural Classification of Proteins) http://scop.mrc-lmb.cam.ac.uk/scop/ 3, comparison of protein structures in 3D http://www.ebi.ac.uk/dali/index.html A, X-ray crystallography B, NMR (nuclear magnetic resonance) The Nobel Prize in Chemistry 1962 Max Ferdinand Perutz John Cowdery Kendrew MRC Laboratory of Molecular Biology Cambridge, United Kingdom NMR and Nobel Price: 1944: I. I. Rabi, suggests that information about atoms nuclei can be obtained by studying the internal magnetism of protons. This forms the fundamental basis for todays resonance imaging technologies 1952: Physicists E. Purcell (Harvard) and F. Bloch (Stanford) discover Nuclear Magnetic Resonance (NMR). 1991: R. Ernst, Advances in NMR could lead to the ability to directly observe the chemical action of medication in the body. 2002: John B. Fenn, Koichi Tanaka, Kurt W thrich for the development of nuclear magnetic resonance spectroscopy for determining the three- dimensional structure of biological macromolecules in solution Kti 11 3D structure comparison: http://www.ebi.ac.uk/dali/index.html 1, Tools to investigate the protein conformation 2, Super secondary structures and domains 3, globular proteins and SCOP 4, fibrous proteins Super secondary structures, also called motifs or simply folds, are particularly stable arrangements of several elements of secondary structure and the connections between them. Two simple and common motifs that provide two layers of secondary structure. Connections between βstrands in layered βsheets Two arrangements of βstrands stabilized by the tendency of the strands to twist (扭曲). Hemolysin (溶血素) (a pore-forming Photolyase(光解酶) (a protein toxin that kill