复合酶法提取石榴籽多糖的工艺优化.PDFVIP

※工艺技术 食品科学 2016, Vol.37, No.18 19 复合酶法提取石榴籽多糖的工艺优化 1 1 1 2 1 1 王占一 ，张立华 ，王玉海 ，戴 博 ，郑丹丹 ，王 飞 （1.枣庄学院生命科学学院，山东 枣庄 277160 ；2.空军总医院药学部，北京 100142 ） 摘  要：探讨应用复合酶法提取石榴籽多糖的最佳工艺条件。以多糖得率为考察指标，在单因素试验基础上，通 D 过 -最优混料试验设计优化复合酶配比，Box-Behnken试验设计优化得出影响因素的最佳参数水平，进而得出最佳 工艺条件。结果显示：复合酶最优配比为果胶酶24.2%、纤维素酶67.2%、甘露聚糖酶8.6%；并以此参数为基础， 得到复合酶法提取石榴籽多糖的最佳工艺条件为液固比20 ∶1 （mL/g ）、介质pH 4.5 、酶解温度48.5 ℃、酶解时间 287 min，在此条件下，石榴籽多糖得率为2.83%。 D 关键词：石榴籽；多糖；复合酶法； -最优混料设计；响应面分析法；提取工艺 Optimization of Enzymatic Extraction of Polysaccharides from Pomegranate Seeds with Mixed Enzymes 1 1 1 2 1 1 WANG Zhanyi , ZHANG Lihua , WANG Yuhai , DAI Bo , ZHENG Dandan , WANG Fei (1. College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China; 2. Department of Pharmacology, General Hospital of Air Force, Beijing 100142, China) Abstract: In this study, we optimized the conditions for enzymatic extraction of polysaccharides from pomegranate seeds with a mixed enzyme preparation consisting of pectinase, cellulase and mannase. The optimization of the mixing ratio of the three enzymes was performed by the combined use of one-factor-at-a-time method and D-optimal mixture design. Thereafter, the enzymatic extraction conditions were optimized by response surface methodology using Box-Behnken design. The yield of polysaccharides was taken as response for both optimizations. It was shown that the optimal combination of enzymes were 24.2% pectinase, 67.2% cellulase and 8.6% mannose and that the optimal extraction c