MR医学影像文献Yan-2016-Adhesion of Shewanella oneidensis MR.pdfVIP

Article pubs.acs.org/est Adhesion of Shewanella oneidensis MR‑1 to Goethite: A Two- Dimensional Correlation Spectroscopic Study Wei Yan,† Hongbo Wang,‡ and Chuanyong Jing*,† †State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China ‡School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China S *Supporting Information ABSTRACT: Bacterial adhesion to mineral surfaces is an important but underappreciated process. To decipher the molecular level process and mechanism, the adhesion of Shewanella oneidensis MR-1 cells to goethite was investigated using flow-cell attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy coupled with two- dimensional correlation spectroscopy (2D-COS) analysis. The FTIR results indicate that bacterial phosphate-moieties play an important role in the formation of mono- and bidentate inner-sphere complexes, whereas carboxylic groups on cell surface only have a minor contribution to its adhesion. The 2D-COS analysis in short-term (0−120 min) and long-term (2−18 h) stages reveal that the adhesion process was in the following sequence: change in H-bonds of proteins on cell surfaces formation of monodentate inner-sphere surface complexes formation of outer-sphere surface complexes transformation of protein secondary structure on cell surfaces formation of additional bridging bidentate surface complexes. In addition, the adhes